【摘要】 建立同时测定鱼肉组织中6种ATP关联物的高效液相色谱方法。采用反相高效液相色谱分离模式,使用Sepax Bio-C18(250mm×4.6mm,5μm,200A。)色谱柱,以60mmol/L磷酸二氢钾、60mmol/L磷酸氢二钾组成的缓冲液(pH6.68)为流动相等比洗脱,流速0.6mL/min;紫外检测波长为254nm,带宽为16nm。6种组分在20min内基线分离,平均加标回收率在98.3%～104.1%之间,线性范围为1～400mg/L,检出限在15～40μg/L之间。该方法简便、准确,易于移植。更多还原

【Abstract】 The method for determination of ATP-related compounds in fish was established.The content of ATP-related compounds in fish were determined by RP-HPLC with a Sepax Bio-C18(250mm×4.6mm ID,5μm,200 A.)column,and UV detector at a wave length of 254 nm(BW 16 nm).Mobile phase was 60 mmol/L KH2PO4 and 60 mmol/L K2HPO4(pH=6.68) and flow rate was 0.6mL/min.The separation was achieved in 20 min.All the calibration curves were linear in the mass concentration range of 1-400 mg/L.The average recoveries of the standard additions were between 98.3%-104.1%,and the LOD were between 15-40μg /L for ATP-related compounds.This method proved to be reliable and reprodcible.更多还原