【摘要】 以荔枝品种‘元红’处于花性分化时期的花蕊为材料,对蛋白提取方法、蛋白样品上样量、凝胶浓度、平衡时间及染色方法等步骤进行优化。采用酚抽法提取蛋白质,用24 cm、pH4~7的IPG胶条,1.2 mg上样量,12%T凝胶,胶条平衡20 min,用考马斯亮蓝法染色,2-DE图谱经ImageMaster 2D Platinum软件分析,可以检测到至少1 200个蛋白点。荔枝雌花双向电泳体系的建立,为开展荔枝花性分化蛋白质组学方面的研究奠定了基础。更多还原

【Abstract】 The two-dimensional electrophoresis(2-DE)technology was used to demonstrate the protein profile of Litchi(Litchi chinensis Sonn.,cultivar Yuanhong)flower buds.The key processes of sample extraction method,sample loading,gel concentrations,equilibrium time and staining methods of 2-DE system were optimized.Proteins were extracted by phenol extraction method.The results showed that more than 1 200 protein spots were detected by Image Master 2D Platinum with the parameters including pH 4~7 24 cm IPG strip,1.2 mg protein sample loading,12%T gel,20 min equilibrium and Coomassie Brilliant Blue(CCB)R250 stain.The optimized system provided the foundation for further proteomics analysis of flower sex-differentiation in Litchi.更多还原