【摘要】 以木薯品种"GR911"、"面包木薯"试管苗叶片为材料,研究木薯枯萎病病原菌粗提物对愈伤组织增殖与分化的影响,以期建立枯萎病病原菌粗提物为选择压力的木薯离体抗病筛选体系。结果表明:所用品种幼叶愈伤诱导与增殖的适宜配方为改良的MS培养基+0.5-0.75mg/L2,4-D;愈伤分化适宜配方为的MS培养基+0.75-1.0mg/LZT。"GR911"与"面包木薯"愈伤增殖过程中,愈伤褐化率达到50%所需的细菌液粗提物浓度分别为5.5×106、2.5×106cfu/mL;愈伤组织分化过程中,愈伤褐化率达到50%所需的菌液粗提物浓度分别为7.0×106、4.0×106cfu/mL。通过建立木薯愈伤组织离体抗病筛选体系,为木薯无性系变异育种奠定基础。更多还原

【Abstract】 This study was made to clarify the effects of bacterial crude toxin culture on proliferation and differentiation of callus in cassava, to screen system of resistance to bacterial wilt caused by Xanthomonas manihotis(Artheodet Bey.)Stars in vitro. The young leaves from plantlet were used as explants. On basal modified MS medium, the optimum hormone concentration is 0.5-0.75 mg/L 2 ,4-D for induction and proliferation of callus, and 0.75-1.0 mg/ L ZT for differentiation of callus. To achieve 50 % callus browning rate during callus proliferation, the concentration of bacteria crude toxin culture in medium should be 5.5×106 cfu/ml for species GR991 and 2.5×106 cfu /ml for species MianBao. To get 50 % callus browning rate during callus differentiation, the concentration of bacteria crude toxin culture in medium should be 7.0×106 cfu /ml for species GR991 and 4.0×106 cfu /ml for species MianBao. By this experiment, the screening system of resistance to bacterial wilt was established in vitro in cassava, and this system can be used for clone breeding studies.更多还原