【摘要】 目的研究DEPDC7基因在正常肝细胞、肝癌细胞及其他肿瘤细胞中的表达情况。方法常规培养正常肝细胞HL-7702,肝癌细胞SMMC7721、HepG2、Huh-7,其他肿瘤细胞ZR-7530、MDA-MB-231、SGC7901、SW480。用MTT检测肝癌细胞转染前后的增殖情况,用RT-PCR方法在转录水平检测DEPDC7基因的表达,用Western blot方法从蛋白质水平检测DEPDC7蛋白的表达。结果 3株肝癌细胞生长增殖速度差异有统计学意义(P<0.05)。肝癌细胞DEPDC7基因在mRNA和蛋白质表达水平比正常肝细胞降低(P<0.05),而其他肿瘤细胞DEPDC7基因表达差异无统计学意义(P>0.05)。结论 DEPDC7基因在正常肝细胞中选择性高表达,而在肝癌细胞株中呈低表达。更多还原

【Abstract】 Objective To study the expression of novel DEPDC7 gene in normal hepatic cells, hepatocellular carcinoma cells, and several other neoplastic cells. Methods The hepatic cell line HL-7702, hepatoma cell lines SMMC7721, HepG2, Huh-7, and other neoplastic cells ZR-7530, MDA-MB-231, SGC7901, and SW480 were used in this study. MTT was used to measure the proliferation of hepatoma cell before and after transfection of DEPDC7. RT-PCR method was used to detect the DEPDC7 gene expression, and Western blot was used to detect the proteinexpression. Results There was a significantly difference in the proliferation rate (P<0.05) among the three hepatoma cell lines before and after transfection (P<0.05). The mRNA and protein expression levels of DEPDC7 gene were decreased in hepatoma cells when compared to the normal hepatic cell (P<0.05). However, the difference in the expression levels was not seen in other neoplasm cells. Conclusion DEPDC7 gene was expressed at a higher level in hepatic cell than the hepatocarcinoma cells.更多还原