【摘要】 目的:研究细胞因子信号转导抑制因子-3(SOCS-3)蛋白在小儿肾母细胞瘤组织中的表达及意义。方法:应用组织微阵列技术结合免疫组化SP法检测58例肾母细胞瘤标本和17例瘤旁对照组织中的SOCS-3的表达状况,并分析其临床病理意义。结果:肾母细胞瘤组织中仅14例SOCS-3呈弱阳性表达,表达率为24.1%(14/58);17例瘤旁组织中SOCS-3蛋白阳性表达率为88.2%(15/17),差异有统计学意义,P<0.01。SOCS-3在肾母细胞瘤组织的表达随着肿瘤的临床分期增加而降低(P<0.05),与肾母细胞瘤的临床分期呈负相关,P<0.01;在组织结构良好型与组织结构不良型SOCS-3蛋白表达(47.6%、10.8%)间差异有统计学意义,P<0.05。结论:SOCS-3的表达下降可能与肾母细胞瘤的发生发展密切相关。更多还原

【Abstract】 OBJECTIVE:To investigate protein expression of SOCS-3 and its clinacal significance in Nephroblastoma.METHODS: Using tissue microarray combination with immunohistochemical measure to investigate the protein expression status of SOCS-3 in 58 cases of Nephroblastoma and 17 cases of adjacent tumor samples,analyzing the relation of expression status of SOCS-3 with clinicopathological factors.RESULTS:The ratio of SOCS-3 protein expression was increased in nephroblastoma tissues 24.1%(14/58) compared to adjacent tumor cases 88.2%(15/17,P<0.01) by using streptavidin-biotin pemixidase(SP) method.Within the specimens of Pca group,the differences were statistically significant among pathological grade and clinical stage(P<0.05).There was statistically significant among FHand LH(P<0.05).There was a negative correlation between the expression of SOCS-3 and clinical grades(P<0.01).CONCLUSION: The low expressions of SOCS-3 may have a role in the development and progress of Nephroblastoma.更多还原