【摘要】 目的:探讨乙酰肝素酶(HPSE)基因启动子区甲基化与膀胱癌临床病理特征的相关性。方法:运用甲基化特异性聚合酶链反应(MSP)法检测27例膀胱癌标本及15例正常膀胱组织中HPSE基因启动子甲基化状态,并分析HPSE基因甲基化状态与不同临床病理特征的关系。结果:59.26%(16/27)膀胱癌组织HPSE基因启动子区发生低甲基化改变,另外2例甚至为完全去甲基化改变;而仅有3例(20.00%)正常膀胱组织HPSE基因启动子区发生低甲基化,χ2=5.999,P=0.014。在17例伴淋巴结转移的患者中,发生HPSE基因低甲基化改变15例(88.23%),而在10例无淋巴结转移的患者中,发生HPSE基因低甲基化改变1例(10.00%)。HPSE低甲基化阳性率随淋巴转移而增高,χ2=12.887,P=0.004。不同性别、年龄、瘤体大小、病理分级和临床分期与HPSE基因低甲基化无明显相关性,P>0.05。结论:HPSE基因启动子区低甲基化为频发事件,提示与膀胱癌患者的不良预后相关,可作为膀胱癌判断预后的生物学标志。更多还原

【Abstract】 OBJECTIVE:To study the heparanase(HPSE) gene promoter hypomethylation and its relation with clinical pathology of bladder cancer.METHODS:Methylation-specific PCR was used to detect the methylation status of HPSE promoter of bladder cancer tissues of 27 patients.Fifteen normal bladder tissues were also tested as control.RESULTS:The hypomethylation rate of baldder cancer and normal bladder tissue were 59.26% and 20.00%,respectively(χ2=5.999,P=0.014).The hepomethylation rate of patients with lymph node metastasis was 15/17(88.23%),and 1/10(10.00%) case was detected hepomethylation in the non-lymph node metastasis group.The hypomethylation rate significantly increased with lymph node metastasis(χ2=12.887,P=0.004).There were no significant associations between the hypomethylation frequencies for HPSE gene and patient gender,age,size of tumor,stages and histologic grades(P>0.05).CONCLUSIONS:HPSE promoter hypomethylation may be a frequent epigenetic event of bladder cancer,and may also involve in tumor progression.The methylation rate of HPSE can be used as a molecular marker to predict the prognosis for bladder cancer.更多还原