【摘要】 目的:西妥昔单抗(C225)对鼻咽癌细胞放疗增敏作用及其相关机制的研究。方法:通过免疫细胞化学法测得EG-FR在鼻咽癌CNE-2Z细胞株中的表达水平,MTT法测出西妥昔单抗在体外细胞实验中的工作浓度,克隆形成实验测定不同处理组的Do、SER值。将细胞分为对照组(N)、单纯照射组(R)、单纯用药组(C)和放疗联合使用西妥昔单抗组(RC)4组,应用流式细胞技术(Annexin V/PI标记)检测西妥昔单抗在体外对鼻咽癌细胞凋亡的影响。结果:免疫细胞化学法检测鼻咽癌CNE-2Z细胞株EGFR呈高表达水平。克隆形成实验测出放疗联合西妥昔单抗组Do低于单纯照射组,SER为1.581 1±0.035 7,P<0.05。放疗联合用药组的细胞凋亡率为(31.9±0.98)%,单纯放疗组的细胞凋亡率为(13.1±0.60)%,单纯用药组的细胞凋亡率为(6.4±0.95)%,对照组的细胞凋亡率为(2.5±0.51)%,放疗联合用药组的细胞凋亡率明显高于单纯处理组和对照组,P<0.05。结论:西妥昔单抗增加了鼻咽癌细胞对放疗的敏感性,其机制可能与其诱导细胞凋亡有关。更多还原

【Abstract】 OBJECTIVE: To investigate the mechanism of enhancement of radiosensitivity by Cetuximab in nasopharyngeal carcinoma.METHODS: The expression of EGFR of nasopharyngeal carcinoma CNE-2Z cell line was tested by immunohistochemistry(IHC) and the working concentration of Cetuximab in vitro was detected by MTT colorimetry.Four groups were as follows: No treatment group(N)、Radiation alone group(R)、Cetuximab alone group(C)、Cetuximab after Radiation group(RC).The working concentration were used to the four groups.The apoptosis-inducing effect of Cetuximab in vitro was detected via flow cytometry(Annexin V/PI labeled).RESULTS: The expression of EGFR of CNE-2Z cell line was positive by IHC.The Do in Cetuximab and radiation group was lower than that in the radiation alone group.The SER of CR group was 1.581 1±0.035 7(P<0.05).The apoptosis rate of radiotherapy and cetuximab group was(31.9±0.98)%,the radiation alone group was(13.1±0.60)%,the cetuximab alone group was(6.4±0.95)% and no treatment group was(2.5±0.51)%.FCM showed more prominent apoptosis induced by cetuximab combined with radiation compared to cetuximab or radiation alone groups(P<0.05).CONCLUSION:Cetuximab can enhance the radiosensitivity of the CNE-2Z cell,which may be associated with apoptosis induction.更多还原