【摘要】 目的:探讨E-cadherin、β-catenin和PTEN在宫颈鳞癌及上皮内瘤变组织中的表达及临床意义。方法:采用免疫组化方法检测75例宫颈鳞癌、30例宫颈上皮内瘤变(CIN)及10例正常宫颈组织中E-cadherin、β-catenin和PTEN的表达。结果:正常宫颈、CINⅠ、CINⅡ～Ⅲ及宫颈鳞癌组织中E-cadherin阳性表达率依次为100.0%、85.7%、50.0%和37.3%;β-catenin异常表达率依次为0、28.6%、62.5%和84.0%;PTEN阳性表达率依次为100.0%、78.6%、56.3%和40.0%,3种蛋白在正常宫颈、CINⅠ的表达与鳞癌中的表达差异均有统计学意义,在正常宫颈与CINⅡ～Ⅲ中的表达差异也均有统计学意义,P<0.05。E-cadherin、PTEN在宫颈鳞癌中的阳性表达均与病理组织学分级、淋巴结转移及临床分期有关,P<0.05;β-catenin异常表达与病理组织学分级、淋巴结转移有关(P<0.05),但与临床分期无关(P>0.05)。宫颈鳞癌组织中E-cadherin与PTEN阳性表达呈正相关,与β-catenin异常表达呈负相关;β-catenin异常表达与PTEN阳性表达呈负相关。结论:E-cadherin与PTEN表达缺失或降低及β-catenin异常表达,均在宫颈鳞癌的发生、发展及淋巴结转移中发挥重要作用,且三者之间关系密切。更多还原

【Abstract】 OBJECTIVE:To investigate the expression and clinical significance of E-cadherin,β-catenin and PTEN in cervical squamous cell carcinoma(CSCC) and cervical intraepithelial neoplasia.METHODS: Expression of E-cadherin,β-catenin and PTEN were examined by using immunohistochemical staining in 75 CSCCs,30 cervical intraepithelial neoplasias(CIN) and 10 normal cervical epithelia.RESULTS: In normal cervical epithelia,CINⅠ,CINⅡ-Ⅲ and CSCC specimens,the positive expression rates of E-cadherin were 100.0%,85.7%,50.0% and 37.3%,respectively;the aberrant high expression rates of β-catenin were 0,28.6%,62.5% and 84.0%;the positive expression rates of PTEN were 100.0%,78.6%,56.3% and 40.0%.There were significant differences among the expression of all of those in normal cervical epithelia,CINⅠand CSCCs(P<0.05),in normal cervical epithelia and CINⅡ-Ⅲ(P<0.05).Positive expression rate of E-cadherin and PTEN were associated with histopathologic grading,nodal metastasis and clinical staging(P<0.05).The aberrant expression rate of β-catenin was associated with histopathologic grading and nodal metastasis(P<0.05),but was not with clinical staging(P>0.05).It was positive correlation that E-cadherin expression was in comparison with PTEN protein expression in CSCCs,but negative correlation with the aberrant expression of β-catenin,and then the latter was negative correlation with PTEN positive expression.CONCLUSIONS: It may have an important role in the carcinogenesis,progression and metastasis of CSCC that there are the deletion and decrease in expressions of E-cadherin and PTEN and that there is an aberrant expression of β-catenin,and that they have close relations among the expression of E-cadherin,PTEN and β-catenin in CSCC.更多还原