【摘要】 目的:检测神经导向分子Semaphorin 5A在胃癌组织中表达,并探讨其在胃癌发生过程中的功能作用。方法:使用反转录多聚酶链反应(RT-PCR)检测Semaphorin 5A在不同胃癌细胞系中的表达,使用Real-time RT-PCR和蛋白质印迹法检测Semaphorin 5A在30例胃癌组织和对应的胃黏膜组织中的表达;采用RNA干扰技术建立稳定Semaphor-in 5A表达抑制胃癌细胞系,使用MTT法、软琼脂克隆形成实验和流式细胞仪分别检测Semaphorin 5A基因沉默后对胃癌细胞增殖、生长和凋亡的影响。结果:Semaphorin 5AmRNA在不同胃癌细胞系中高表达,Semaphorin 5A在30例胃癌组织中的表达水平明显高于对应的正常胃黏膜组织,P<0.05;Semaphorin 5A表达沉默后能够抑制胃癌细胞的增殖、克隆性生长和促进胃癌细胞的凋亡。结论:Semaphorin 5A在胃癌组织中高表达,通过增强胃癌细胞的增殖、克隆性生长能力和抑制胃癌细胞的凋亡在胃癌的发生过程中发挥着重要作用。更多还原

【Abstract】 OBJECTIVE: To examine the expression of Semaphorin 5A in gastric cancer and explore its functional roles in gastric carcinogenesis.METHODS: The expression of Semaphorin 5A was examined by reverse transcription-polymerase chain reaction(RT-PCR) analysis in different gastric cancer cell lines and detected by real-time RT-PCR and western blotting in 30 pairs of primary gastric cancer and normal gastric mucosa tissues.Semaphorin 5A downregulation gastric cancer cell line was generated by RNA interfere technique(RNAi) and the effect of Semaphorin 5A-silencing on the growth,proliferation and apoptosis of gastric cancer cell was tested by using MTT,anchorage-independent growth as well as flow cytometry assays respectively.RESULTS: Semaphorin 5A was overexpressed in all human gastric cancer lines and the expression of Semaphorin 5A was significantly higher in cancer tissues than that in normal mucosa tissues(P<0.05).siRNA mediated Semaphorin 5A knockdown significantly suppressed the proliferation and anchorage-independent growth,and induced the apoptosis of gastric cancer cell lines.CONCLUSION: Semaphorin 5A expression is upregulated in gastric cancer,the extent of which is positively correlated to the gastric carcinogenesis by promoting gastric cell growth,proliferation and inhibiting gastric cancer cell apoptosis.更多还原