【摘要】 目的:探讨结直肠癌组织中E-cadherin和β-catenin蛋白的表达与临床病理的相关性及意义。方法:应用免疫组织化学染色检测56例结直肠癌组织和56例癌旁组织中E-cadherin、β-catenin蛋白的表达。结果:E-cadherin蛋白在结直肠癌组织中的表达明显低于癌旁组织,P=0.0000;低分化癌E-cadherin蛋白的表达低于高分化和中分化癌,P=0.0000;浸润至或侵出浆膜层的癌E-cadherin蛋白的表达低于浸润至肌层的癌,P=0.000 8;有肿瘤出芽者E-cadherin蛋白的表达低于无肿瘤出芽者,P=0.006 1;淋巴结转移组E-cadherin蛋白的表达低于无淋巴结转移组,P=0.0000。β-catenin蛋白在结直肠癌组织中的表达低于癌旁组织,P=0.036 9;低分化结直肠癌组织β-catenin蛋白的表达低于高分化和中分化结直肠癌组织,P=0.023 8;浸润至或侵出浆膜层的结直肠癌组织β-catenin蛋白的表达低于浸润至肌层的结直肠癌组织,P=0.020 2;有肿瘤出芽的结直肠癌组织β-catenin蛋白的表达低于无肿瘤出芽的结直肠癌组织(P=0.025 7);有淋巴结转移的结直肠癌组织β-catenin蛋白的表达低于无淋巴结转移的结直肠癌组织(P=0.013 3)。结论:结直肠癌组织E-cadherin和β-catenin蛋白表达状况的变化标志着癌细胞在形态-分子表型上发生了改变,表达丢失时即意味着出现了上皮-间叶转化(EMT),后者预示着肿瘤趋向浸润和转移。E-cadherin和β-catenin在肿瘤发展的过程中发挥着不同的作用,因而调控E-cadherin和β-catenin的表达有望成为肿瘤的个体化干预靶点。更多还原

【Abstract】 OBJECTIVE: To explore the significance and correlation between colinicopathologic features and expression of E-cadherin and β-catenin in colorectal cancer tissues.METHODES: The immunohistochemical staining for E-cadherin and β-catenin were performed in 56 cases of primary colorectal cancerous tissues and 56 cases of paracancerous tissues.RESULTS: The positive rate of E-cadherin expression was significantly lower in cancerous tissues than those in paracancerous tissues(P=0.000 0).The expression of E-cadherin was lower in poorly-differentiated cancer tissues than those in well and moderate-differentiated cancer tissues(P=0.000 0).The expression of E-caherin was lower in cancers with tumor infiltrating into or beyond serous layer than those in cancers with infiltrating into lamina muscularis(P=0.000 8).It was lower in the colorectal cancer tissues with tumor budding than those in without tumor budding(P=0.006 1),and it was lower in the colorectal cancer tissues with lymph node metastasis than those in without lymph nodes metastasis(P=0.000 0).The positive rate of β-catenin expression was significantly lower in the colorectal cancer tissues than those in paracancerous tissues(P=0.036 9).The expression of β-catenin was lower in poor differentiated cancer tissues than those in well and moderate-differentiated cancer tissues(P=0.023 8).The expression of β-catenin was lower in cancers with infiltrating into or beyond serous layer than those in cancers with infiltrating into lamina muscularis(P=0.020 2),and it was lower in the colorectal cancer tissues with tumor budding than those in without tumor budding(P=0.025 7).It was lower in the colorectal cancer tissues with lymph node metastasis than those in without lymph nodes metastasis(P=0.013 3).CONCLUSIONS: The differential expression profiles of E-cadherin and β-catenin represents that there is a transformation of morphologic-molecular phenotype of cancer cell in colorectal cancer tissues.These protein expression loss contributes to occurrence of epithelial-mesechymal transition,and which indicates that the tumor acquires potential to invasiveness and metastasis.The E-cadherin and β-catenin play a different role in the development of cancer,and that they are interrelated and coordinated one another,and therefore to modulate the expression of E-cadherin and β-catenin,which is expected to become an individual intervention targets for the tumor.更多还原