【摘要】 目的:探讨芥菜籽(MS)对1,2-二甲基肼(DMH)诱导的大鼠大肠癌的预防作用。方法:用DMH腹腔注射诱导大鼠发生大肠癌,观察MS对诱癌率及胸腺指数和脾指数等的影响。结果:用DMH成功诱导大鼠大肠癌模型,正常对照组未见肿瘤发生,DMH组腺癌发生率为83.33%(10/12),腺瘤发生率为16.67%(2/12),总肿瘤发生率为100%(12/12),肝及淋巴结转移率为58.33%(7/12)。5%MS和7.5%MS组腺癌发生率比DMH组分别降低了58.33%和75.00%(χ2=15.662,P=0.000),肝及淋巴结转移率分别降低了41.66%和50.00%(χ2=8.585,P=0.014),总肿瘤发生率〔66.67%(8/12),41.70%(5/12)〕亦显著低于DMH组(χ2=9.687,P=0.008),而腺瘤发生率〔41.67%(5/12),33.33%(4/12)〕则高于DMH组。7.5%MS组大鼠胸腺指数和脾指数均明显大于DMH组,P值分别为0.013和0.037;同时5%MS组胸腺指数也显著大于DMH组,P=0.039。结论:MS对DMH诱导的大肠癌有预防发生及减少转移的作用,并能改善机体免疫功能。更多还原

【Abstract】 OBJECTIVE: To observe the effect of mustard seed(MS) against colorectal cancer in rats induced by 1,2-dimethylhydrazine(DMH) in vivo.METHODS: The colorectal cancer model induced by DMH intraperitoneal injection.The effect of mustard seed on the incidence of colorectal cancer,spleen index and thymus index were observed.RESULTS:The colorectal cancer model was successfully established in this experiment.The incidence of colorectal adenocarcinoma in DMH group was 83.33%(10/12),the adenoma incidence was 16.67%(2/12),the total tumor incidence was 100%(12/12),and the liver and mesenteric lymph node metastasis rate was 58.33%(7/12).The incidence of adenocarcinoma in 5% MS group and 7.5%MS group was 58.33% and 75.00% respectively lower than that in DMH group(χ2=15.662,P=0.000).The liver and mesenteric lymph node metastasis rate of colorectal cancer in 5% MS group and 7.5%MS group was 41.66% and 50.00% respectively lower than that in DMH group(χ2=8.585,P=0.014).And the incidence of total colorectal tumor in 5% MS group and 7.5%MS group was 66.67%(8/12) and 41.70%(5/12) respectively lower than that in DMH group(χ2=9.687,P=0.008).While the incidence of colorectal adenoma in 5% MS group(41.67%,5/12) and 7.5%MS group(33.33%,4/12) was slightly higher than that in DMH group(16.67%,2/12).The thymus and spleen index of rats in 7.5%MS group were markedly higher than that in DMH group(P=0.013;P=0.037).And the thymus index of rats in 5%MS group was also markedly higher than that of DMH group(P=0.039).CONCLUSION: MS have the effects of preventing colorectal cancer induced by DMH,and it can improve organism’s immunefunction.更多还原