【摘要】 目的:研究肝癌缺失基因1(DLC-1)和MMP-2、MMP-9在肝癌组织中的表达及其与临床病理分期的关系,为探讨对肝癌发生、发展及转移的分子机制提供依据。方法:采用免疫组化ABC法检测50例肝癌和26例肝脏良性病变肝血管瘤及其周围正常组织中DLC-1和MMP-2、MMP-9的表达情况,结合临床病理资料分析二者在肝癌中表达的意义。结果:DLC-1在原发性肝癌、肝血管瘤组织和周围正常组织中的表达率分别为31.2%,72.1%和81.7%(P<0.01),MMP-2在3组中的表达率为77.1%,33.3%和26.7%(P<0.05),MMP-9的表达率为74.3%,29.5%和23.7%(P<0.05)。DLC-1和MMP-2、MMP-9在原发性肝癌组织中的表达呈明显负相关(Kappa值为-0.459,-0.523),DLC-1和MMP-2、MMP-9与肝癌的发生、分期和淋巴结转移关系密切(P<0.01)。结论:DLC-1低表达或无表达和MMP-2、MMP-9高表达与肝癌的发生发展转移有关,DLC-1和MMP-2、MMP-9在影响肝癌发生、发展、转移方面存在一定的协同效应,DLC-1有可能成为肝癌早诊和预后判断的候选标志物。更多还原

【Abstract】 Objective: To analyze the expression of deleted in liver cancer 1(DLC-1) and MMP-2、MMP-9 in hepatocellular carcinoma to further understand the occurrence,development and transfer of the molecular mechanisms of liver cancer.Methods: Immunohistochemistry was employed to determine the protein level of DLC-1and MMP-2、MMP-9 in 50 hepatocellular carcinoma,26 hepatic hemangiomas and the adjacent normal liver tissues.Results: The positivity rates of DLC-1 differed significantly between hepatocellular carcinoma,hepatic hemangiomas and normal tissues(31.2%,72.1% and 81.7%,respectively,P<0.001).The positivity rates of MMP-2 in the 3 tissues were 77.1%,33.3% and 26.7%(P<0.05),MMP-9 were 74.3%,29.7% and 23.7%(P <0.05),also showing significant differences(P<0.001).The aberrant expression of DLC1 showed an inverse correlation to MMP-2、MMP-9(κ=-0.4591,-0.523).Conclusion: The abnormal expression of DLC1 and MMP-2、MMP-9 might participate in the occurrence progression,and metastasis.But there was an obviously synergistic effect between DLC-1 and MMP-2、MMP-9.Therefore,DLC-1 may be the candidate markers of early diagnosis and prognosis of hepatocellular carcinoma.更多还原