【摘要】 [目的]研究黄皮甲醇提取物对稗草的除草活性及其除草有效成分。[方法]采用硅胶柱层析分离及活性跟踪的分离方法。[结果]生物测定结果表明:黄皮甲醇提取物对油菜、萝卜和稗草有很好的生物活性,表明其有效成分为anisolactone和2’,3’-epoxyanisolactone,暂定为黄皮素内酯Ⅰ和黄皮素内酯Ⅱ。毒力测定结果表明:在80 mg/L时,对稗草根长、茎长和鲜质量的抑制率分别为96.00%、84.71%和89.85%。且回归方程的相关系数均达到0.99以上。[结论]黄皮甲醇提取物对稗草有很好的防治效果。更多还原

【Abstract】 To study on the herbicide activity and active constituent of the methanolic extract of Clausena lansium(Lour.) Skeels.[Methods] By separating with silica column chromatography and TLC methods,guided by bioassay of herbicidal activity.[Results] Bioassay revealed that the leaf of C.lansium(Lour.) Skeels was very excellent against E.crusgalli(L.) Beauv.,two constituents were isolated from C.lansium,they were identified as anisolactone and 2’,3’-epoxyanisolactone by spectroscopic methods.Bioassay results revealed that at the concentration of 80 mg/L,the inhibition rate of 2’,3’-epoxyanisolactone against root growth,shoot growth and fresh weight of E.crusgalli(L.) Beauv were 96.00,84.71 and 89.85% respectively.[Conclusions] The methanolic extract of C.lansium(Lour.)Skeels has good effect on E.crusgalli(L.).更多还原