【摘要】 目的:检测MCM5蛋白和PCNA在肺癌组织中的表达,探讨两者与肺癌各临床病理因素之间的关系及两者相互的联系,从而为评估肺癌的发生发展、预后及治疗提供理论依据。方法:运用免疫组织化学技术分别检测MCM5蛋白和PCNA在68例肺癌组织和20例正常组织中的表达情况,分析其与临床病理因素之间的关系及两者相互的联系。结果:MCM5表达的阳性信号位于细胞核,胞浆无着色。在正常肺组织中,MCM5的表达局限在上皮基底部的1/3至1/2的细胞,在肺癌组织中,MCM5的表达分布广泛,靠近上皮表面的细胞也可见大量表达。(1)正常肺组织和肺癌组织的MCM5的表达的差别有统计学意义(P<0.01)。(2)肺癌中MCM5表达与分化程度、淋巴结转移有显著相关性(P<0.05),与病人年龄、性别无显著相关性(P>0.05)。(3)PCNA的表达与肺癌的分化程度无显著相关性(P>0.05)。(4)在肺正常组织中,PCNA标记指数高于MCM5标记指数,两者具有显著差异性(P<0.05)。在肺癌组织中,PCNA与MCM5标记指数无显著差异性(P>0.05)。结论:(1)在肺组织中,微小染色体维持蛋白5(MCM5)是一种可靠的细胞增殖标志物。根据MCM5染色的组织结构差异和平均光密度能比较准确区分肺正常组织和癌组织。MCM5表达与肺癌的分化程度显著相关,因此MCM5表达可以提示肺癌的恶性程度,有助于临床判断病人的预后以及选择合适的治疗方法。MCM5与其他增殖标志PCNA相比是一种更好的细胞增殖标志物,是肺癌细胞的更好标志和分级指标。(2)PCNA在肺癌组织中过表达,提示PCNA的过表达与肺癌的发生发展关系密切。更多还原

【Abstract】 Objective:To detect the expression of MCM5 protein and PCNA in lung cancer tissues,and to evaluate the relationship between their expressions and clinical pathological parameters and their mutual correlation,and further provide theoretical basis for evaluating the occurrence,development,prognosis and treatment of lung cancer.Methods:Immunohistochemical technique was used to detect the expression of MCM5 protein and PCNA in 68 cases of lung cancer tissues and 20 cases of normal tissues,then to analyze the relationship between the expressions and clinical pathological parameters.Results:Positive signals of MCM5 expression lacates at nucleus,but not kytoplasm.Expression of MCM5 located limitedly in 1/3 to 1/2 cells at basilar part of epithelium in normal epithelial tissues,but that was expressed widespread in lung cancer tissues and a large number of such expressions were near to the surfaces of epithelium.①The expression of MCM5 was significantly different in lung cancer tissues and that in tumor adjacent tissues(P<0.05).② The expression of MCM5 was closely correlated with differentiation degrees and lymphaden(P<0.05),but were not closely correlated with ages and gender of patients(P>0.05).③There was no significant correlation between the expression of PCNA and differentiation degrees(P>0.05).④For normal tissues,PCNA labeling index was significantly higher than that of MCM5(P<0.05).For lung cancer tissues,PCNA labeling index and MCM5 labeling index was not significantly different(P>0.05).Conclusions:①The differences of tissue structure based on MCM5 staining and average optical density can comparatively distinguish normal lung tissues and cancer tissues.The expression of MCM5 was significantly correlated with differentiation degrees of lung cancer,hence expression of MCM5 can suggest malignant degree of lung cancer,contribute to judge prognosis and help doctors choose suitable treatment methods.Comparing with other proliferating labeling PCNA,MCM5 was a better cell proliferating marker and was more significantly correlated with differentiation degrees of tumors,and was a better marker and ranking index for lung cancer cells.②PCNA was overexpressed in lung cancer,which suggests that the overexpression of PCNA was closely correlated with occurrence and development of lung cancer.更多还原