【摘要】 目的:对不同周龄的Fmr1(fragile X mental retardation 1)基因敲除和野生型雄性小鼠睾丸组织诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)的表达进行分析比较,探讨Fmr1基因敲除小鼠睾丸组织中iNOS表达的差异,为脆性X综合征的研究提供背景资料。方法:不同周龄(4、6、8、10周)的Fmr1基因敲除型和野生型雄性小鼠各6只,先采用PCR技术对基因敲除和野生型小鼠进行基因型鉴定,之后所有小鼠麻醉取睾丸组织,采用石蜡包埋切片免疫组化染色技术对基因敲除和野生型小鼠睾丸iNOS的表达进行检测并作对比分析。结果:iNOS在4周野生型小鼠睾丸间质细胞呈弱阳性表达,在6周小鼠呈阳性表达,在8周和10周小鼠呈强阳性表达,且基因敲除小鼠睾丸的iNOS表达均弱于野生型小鼠。结论:Fmr1基因敲除小鼠在缺失FMR1蛋白(FMRP)后的睾丸组织中iNOS的表达降低。更多还原

【Abstract】 Objective: To analyze the expression of inducible nitric oxide synthase(iNOS) in the testis tissues of Fmr1(fragile X mental retardation 1) knockout and wild-type male mice in different developmental stages,and provide background information for researches on fragile X syndrome.Methods: This study included 4,6,8 and 10 weeks old Fmr1 knockout and wild-type male mice,6 in each age group.We identified the genotype of the mice by PCR,and detected and compared the expression of iNOS in the testis tissues of the Fmr1 knockout and wild-type mice by immunohistochemistry.Results: The iNOS expression was weakly positive in the Leydig cells of the 4-week-old mice,moderately positive in the 6-week-old ones,and strongly positive in 8-and 10-week-old ones,significantly weaker in the Fmr1 knockout than in the wild-type ones.Conclusion: The expression of iNOS significantly decreases in the testis of Fmr1 knockout mice,suggesting that iNOS may be involved in the pathogenesis of fragile X syndrome.更多还原