【摘要】 目的:探讨雌激素受体(estrogen receptors,ERs)在帕金森病(Parkinson’s disease,PD)中的作用特点。方法:应用经典化学毒素MPTP腹腔注射制备小鼠PD模型,WT、αERKO和βERKO雄性小鼠随机分为Saline组和MPTP组,爬竿试验观察行为学改变,荧光定量PCR法检测纹状体和中脑ERα、ERβ、TH、DAT和VMAT2 mRNA表达的变化。结果:与WT相比,αERKO小鼠中多巴胺缺失程度更大,爬竿试验显示αERKO小鼠更早出现行为学异常。αERKO和βERKO小鼠中脑TH、DAT和VMAT2 mRNA表达均降低。WT小鼠MPTP染毒致PD模型后,纹状体和中脑ERαmRNA表达增加,ERβmRNA表达降低。MPTP致PD模型后,αERKO和βERKO小鼠TH和DAT mRNA表达变化情况与WT小鼠不同,βERKO小鼠VMAT2 mRNA表达变化情况与WT小鼠相同,但αERKO小鼠VMAT2 mRNA表达变化情况与WT小鼠不同。结论:ERs在黑质纹状体DA系统中有神经保护作用,ERα亚型在此过程中发挥着更重要的作用。更多还原

【Abstract】 Objective:Estrogen-mediated neuroprotection in the nigrostriatal dopamine system,depend on the estrogen receptors(ERs),is now widely accepted.In the present study,the role of the two ERs in MPTP toxicity was investigated.Methods:Adult male wild type(WT),ERs knock out(αERKO and βERKO) mice were treated with MPTP.To determine the degree of bradykinesia,pole test was performed.Quantitative real time PCR was used to examine selective genes of the dopaminergic system.Results:Our data demonstrated that administration of 1-methyl-4-phenyl-1,2,3,6-tetrapyridine(MPTP,11 mg / kg) to WT mice led to depletion of DA,DOPAC and HVA.The degree of DA depletion in the αERKO mice was greater than WT mice.Behavioral testing showed that MPTPinjected mice exhibited motor deficits in the pole test.After 2 days of MPTP treatment,a significant prolongation of T-LA was observed in αERKO mice.We also observed that mRNA expression of ERα was significantly increased in the striatum and midbrain in MPTP-treated animals,while the expression of ERβ decreased.The expression of tyrosine hydroxylase(TH),dopamine transporter(DAT) and vesicular monoamine transporter subtype 2(VMAT2) mRNA were decreased in αERKO and βERKO mice.The downregulation of TH and DAT mRNAs by MPTP in the midbrain acted on both ERα and ERβ,whereas MPTP treatment lead to decreased mRNA expression of VMAT2 via ERα.Conclusion:Our results suggested that estrogen acting via ERs is an important regulative factor for the nigrostriatal dopamine system,ERα may play a more significant role.更多还原