【摘要】 目的:探讨Y微缺失与同源染色体联会异常在精子发生障碍中的意义。方法:选择无Y微缺失的单纯精子发生障碍患者3例、合并有Y微缺失和AZFc缺失的精子发生障碍患者3例、无Y微缺失的精子发生正常者(3例)为对照组;利用免疫荧光技术比较3组睾丸组织中精母细胞的同源染色体联会情况,包括联会复合体(synaptonemal complexes,SC)上gap和split出现的数目。结果:SC中出现gap的频率在对照组为10%、单纯精子发生障碍组为22%、合并有Y微缺失的精子发生障碍组为25%。两个精子发生障碍组与对照组相比均有显著性差异(P<0.05),两个精子发生障碍组之间无统计学差异。SC中出现split的频率对照组为1%、单纯精子发生障碍组为13%、合并有Y微缺失的精子发生障碍组为15%。两个精子发生障碍组与对照组相比均有显著性差异(P<0.01),两个精子发生障碍组之间无统计学差异。结论:精母细胞中同源染色体联会异常是影响精子发生的重要机制之一,Y微缺失的睾丸组织精母细胞中同源染色体联会异常有加重的趋势。更多还原

【Abstract】 Objective:To investigate the influence of Y chromosome microdeletion and homologous synapsis abnormalities in spermatocytes of male spermatogenesis failure.Methods: Nine cases were recruited in this study,including controls(3 cases),with normal spermatogenesis in their testes;spermatogenesis failure group(3 cases),with spermatogenesis arrest in their testes and without Y microdeletion;and spermatogenesis failure with Y microdeletion group(3 cases).Immunofluorescent staining technology was used to analyze synaptonemal complexes(SC) of spermatocytes.Results: There were 10%,22%,25% gaps presented in testes of controls,spermatogenesis failure group and spermatogenesis failure with Y microdeletion group,respectively.There were significant differences between two spermatogenesis failure groups and controls(10% vs.22%,P = 0.012;10% vs.25%,P = 0.014).However,there was no significant difference between two spermatogenesis failure groups.There were 1%,13%,15% splits presented in testes of controls,spermatogenesis failure group and spermatogenesis failure with Y microdeletion group,respectively.There were significant differences between two spermatogenesis failure groups and controls(1% vs.13%,P = 0.000;1% vs.15%,P = 0.000).However,there was also no significant difference between two spermatogenesis failure groups.Conclusion: Homologous synapsis abnormalities may play an important factor in spermatogenesis failure.Furthermore,homologous synapsis abnormalities would be more frequent in infertile men with Y chromosome microdeletion.更多还原