【摘要】 目的:探讨原发性高血压(essential hypertension,EH)患者单核细胞中经典瞬时受体电位通道3(transient receptorpotential canonical channel 3,TRPC3)转录水平与单核细胞中白细胞介素1β(interleukin-1β,IL-1β)和肿瘤坏死因子-α(tumornecrosis factor-α,TNF-α)的转录水平以及外周血单核细胞趋化蛋白1(monocyte chemotactic protein 1,MCP-1)浓度的关系。方法:抽取20例原发性高血压患者和20例血压正常的健康体检者的静脉血,分离获得单核细胞,运用实时定量聚合酶链反应法(re-al-time PCR)分别检测外周血单核细胞中TRPC3、TNF-α和IL-1β的mRNA,用酶联免疫吸附法(enzyme linked immunosorbentassay,ELISA)检测外周血MCP-1浓度。结果:与血压正常对照组比较,原发性高血压组单核细胞中TRPC3、TNF-α和IL-1β的mRNA表达升高,MCP-1浓度增高,具有统计学差异(P<0.05)。单核细胞中TRPC3的mRNA水平与TNF-α和IL-1β的mRNA水平以及MCP-1浓度呈现正相关。结论:原发性高血压作为一个慢性炎症过程,单核细胞中TRPC3表达与细胞因子水平相关,TRPC3表达上调可被认为是单核细胞活化的指标之一。更多还原

【Abstract】 Objective:To investigate the relationship of transient receptor potential canonical channel 3(TRPC3) transcription and mRNA levels of tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) in monocytes,and monocyte chemoattractant protein 1(MCP-1) level in peripherial blood of patients with essential hypertension(EH).Methods: The levels of TRPC3,TNF-α and IL-1β mRNA in monocytes of 20 patients with essential hypertension and 20 normotensive controls were analyzed by real-time PCR,the concentration of MCP-1 was measured by enzyme linked immunosorbent assay(ELISA).Results: The elevated TRPC3,TNF-α,IL-1β mRNA levels in monocytes and MCP-1 protein in peripherial blood were observed in patients with essential hypertension.There were significant differences between the two groups(P < 0.05).The level of TRPC3 mRNA was positively correlated with the levels of TNF-α,IL-1β mRNA and MCP-1 protein.Conclusion: Essential hypertension is condsidered as a chronic inflammation process.The expression of TRPC3 in monocytes is related to cytokines.Overexpression of TRPC3 may be regarded as one of indicators of activations of monocytes.更多还原