【摘要】 通过梯度增加种子培养基中甘油浓度驯化氧化葡萄糖酸杆菌（Cluconobater oxydans）,有效地提高了该菌对甘油的耐受性,菌株产二羟丙酮水平比驯化前提高了29%,在此基础上,采用单因素试验,对其发酵工艺进行优化。考察甘油质量浓度、酵母膏质量浓度、CaCO₃质量浓度、接种量对发酵的影响,结果显示最佳工艺条件为:甘油质量浓度为60 g/L,酵母膏质量浓度为5 g/L,CaCO₃质量浓度为10 g/L,接种量4%,在此条件下,经72 h摇瓶发酵甘油转化率达96%,二羟丙酮产量可达57.4 g/L,7.5 L发酵罐分批发酵54 h时甘油转化率达97%,二羟丙酮产量可达58.2 g/L。更多还原

【Abstract】 The glycerol tolerance of the Gluconobater oxydans was improved by adding glycerol to the gradiently seed medium,and the dihydroxyacetone yield was about 29%higher than before taminged.The fermentation conditions were further optimized with one factor experimental design.The effects of glycerol concentration,the amount of yeast extract,CaCO₃ concentration and the inoculum size on the fermentation were studied.The results showed that the optimal conditions were as follows:glycerol of 60 g/L,yeast extract of 5 g/L,CaCO₃ of 10 g/L,and inoculum size of 4%.Under the conditions,the transformation rate of glycerol was 96%and the dihydroxyacetone yield reached 57.4 g/L in shaker flask for fermenting 72 h. The transformation rate of glycerol reached 97%and the dihydroxyacetone yield 58.2 g/L after being cultivated in 7.5 L fermentor for fermenting 54 h.更多还原