【摘要】 以斑马鱼体内卵黄蛋白原(Vtg)作为雌激素污染物的生物标志物,比较研究了不同浓度2,4’-DDT对成年斑马鱼生长、发育、繁殖以及体内Vtg含量的影响。结果表明,当水环境中ρ(2,4’-DDT)为2和10μg·L-1时,斑马鱼产卵量和受精率与空白和溶剂对照组相比均显著下降(P<0.05)。当水环境中ρ(2,4’-DDT)为0.2、2和10μg·L-1时,斑马鱼肝脏指数与空白和溶剂对照组相比显著升高(P<0.05);10μg·L-12,4’-DDT处理组雄性斑马鱼体内Vtg含量显著高于空白和溶剂对照组(P<0.05),而0.2、2和10μg·L-12,4’-DDT处理组雌性斑马鱼体内Vtg含量均显著高于空白和溶剂对照组(P<0.05),且随暴露浓度的增加而增大。SDS-PAGE检测及免疫印迹分析均显示雄鱼血液内出现Vtg特异条带,表明2,4’-DDT对斑马鱼具有雌激素效应。更多还原

【Abstract】 Using zebrafish′s vitellogenin as a biomarker of estrogenic pollutants,a comparative study was carried out to determine impacts of 2,4′-DDT,varying in concentration,on growth,development,reproduction and vitellogenin in vivo.Results show that in water containing 2 and 10 μg·L-1 of 2,4′-DDT,the egg yield and egg fertilization rate of fish decreased significantly,as compared with the blank group(P<0.05).In water containing 0.2,2,and 10 μg·L-1 of 2,4′-DDT,the fish increased significantly in its hepaticosomatic index(P<0.05);A significant increase was observed in vitellogenin level of male fish treated with 10 μg·L-1 2,4′-DDT(P<0.05),and of female fish treated with 0.2,2,and 10 μg·L-1 2,4′-DDT(P<0.05).And the increase got more significant with the exposure increasing in concentration.SDS-PAGE and Western blotting analysis revealed that a Vtg fragment appeared in the blood of male fish,which indicates that 2,4′-DDT has estrogenic effects on zebrafish.更多还原