【摘要】 目的探讨干酪乳杆菌(Lactobacillus casei,L.casei)胞外多糖影响树突状细胞迁移的机制。方法利用细胞因子诱导的方法获得骨髓来源树突状细胞(BMDCs)。体外实验为干酪乳杆菌胞外多糖(EPS)处理DCs 24 h后,采用双抗体夹心ELISA法检测细胞培养上清液中趋化因子受体CCR6和CX3CR1的数量;体内实验为胞外多糖腹腔注射Balb/c小鼠12 h后用采用双抗体夹心ELISA法检测小鼠血液中趋化因子FKN和MIP-3α的分泌量。结果体外实验中,经胞外多糖处理的DCs受体CCR6和CX3CR1数量增加极显著(P<0.01),并且与胞外多糖的浓度呈剂量依赖性;体内实验中,经胞外多糖处理的小鼠血液中FKN和MIP-3α含量增加极显著(P<0.01),并且在50 mg/kg注射量时达到最大。结论胞外多糖能够增加血清中FKN和MIP-3α的含量,并能增加BMDCs受体CCR6和CX3CR1的数量。更多还原

【Abstract】 This study aimed to explore the impact of exopolysaccharide from Lactobacillus casei on migration mechanism of dendritic cells.Murine bone-marrow-derived dendritic cells(BMDCs) were acquired by cytokine induction method.In vitro,Lactobacillus casei exopolysaccharide(EPS) was added to BMDCs culture supernatant on day 6 and cultured for 24 h,then the content of CCR6 and CX3CR1 in the culture supernatant were determined by sandwich enzyme-linked immunosorbent assay.In vivo,mice were treated by intraperitoneal injection of EPS,and 12 hours later,the content of FKN and MIP-3α in serum was determined by sandwich enzyme-linked immunosorbent assay.We found EPS could increase the production of CCR6 and CX3CR1 in vitro(P < 0.01),and with EPS concentration increasing,the secretion had a clear upward trend.EPS could increase the content of FKN and MIP-3α in the serum of mice in vivo(P < 0.01),and when EPS concentration was 50 mg/kg,the content of FKN and MIP-3α in serum achieved maximum.We concluded that EPS could increase the content of FKN and MIP-3α in serum,meanwhile,could increase the content of CCR6 and CX3CR1 in culture supernatant.更多还原