【摘要】 目的:探讨IL-32在增生性瘢痕和瘢痕疙瘩的形成过程中所起的生物学作用。方法:收集2009年10月至2011年6月广东医学院附属医院整形外科手术切除的瘢痕疙瘩组织12例,增生性瘢痕组织12例,正常皮肤24例,分别应用免疫组织化学技术、RT-PCR和Western Blot检测IL-32在它们中的表达情况。结果:IL-32在正常皮肤增生性瘢痕和瘢痕疙瘩组织中均有表达,在正常皮肤中表达较强,在增生性瘢痕及瘢痕疙瘩组织中表达较弱;IL-32mRNA和IL-32蛋白在增生性瘢痕和瘢痕疙瘩中的表达较正常皮肤明显降低(P均<0.05),而增生性瘢痕组和瘢痕疙瘩组之间差异无统计学意义(P>0.05)。结论:IL-32在病理性瘢痕组织的形成过程中起着一定的重要作用,有进一步研究的价值。更多还原

【Abstract】 Objective To explore the biological contribution of IL-32 in the formative course of hypertrophic scar and keloid.Methods From October 2009 to June 2011,12 cases of keloid tissue;12 cases of hypertrophic scar tissue;24 cases of normal skin were collected at Guangdong Medical College Hospital plastic surgical.Take normal skin as control group,the IL-32 mRNA of tissue are investigated in the specimens from normal skin,hypertrophic scar and keloid tissues using reverse transcription polymerase chain reaction(RT-PCR).Further immunohistochemical technique and western blot are performed to detect the expression and distribution of IL-32 protein in the paraffin embedded tissues samples of normal skin,hypertrophic scar and keloid.Results The expression of IL-32 mRNA was obviously lower in hypertrophic scar and keloid tissues compared with those of normal skin respectively.The result of immunohistochemical technique and Western Blot: the expression of IL-32 protein were both lower in hypertrophic scar and keloid tissues compared with those of normal skin respectively(P<0.05).Conclusion The results indicated that IL-32 played an important role in the proliferation of fibroblasts and in the development of pathological scar,the value of further research.更多还原