【摘要】 目的:建立眼角膜成纤维细胞体外培养的方法。方法:采用全角膜组织培养法培养兔角膜成纤维细胞。结果:在培养皿中培养1天后细胞开始贴壁,6天有新生细胞分出,形成梭形的成纤维细胞。传代细胞经4-6天可形成密集的成纤维细胞,细胞呈纤维状,呈漩涡形排列,排列整齐,局部细胞相互平行,第4、5代生长情况良好,细胞生长旺盛,细胞呈密集漩涡形排列。结论:眼角膜成纤维细胞全角膜组织培养法是可行的。更多还原

【Abstract】 Objective:To investigate the culture technique for corneal fibroblast.Methods:The rabbits with corneal fibroblast were cultured by rabbit’s corneal materials.Result:The rabbit entire corneal adhered in 1 day,the cells could be seen climbing out from the corneal limbus in 6 days.The corneal fibroblast of rabbit formed a monolayer and p2 had a fibriform arragement after 4~6 days culture in culture flasks.The p4,p5 cells grew very well in culture flasks and had a fibriform arrangement.Conclusion:Corneal fibroblast from rabbit can be cultured by the entire corneal tissue-culture successfully.更多还原