【摘要】 目的验证MPEG-PLGA纳米胶囊在真核质粒转染细胞中应用的可行性。方法利用直接溶解法将MPEG-PLGA溶解于4℃双蒸水中,加入绿色荧光载体混匀后静置于37℃恒温箱中30 min制成MPEG-PLGA/pEGFP-C3纳米胶囊复合物,检测复合物粒径大小并转染机体细胞,通过对机体细胞绿色荧光的表达判断复合物的转染效率。结果 MPEG-PLGA/pEGFP-C3粒径为28.3 nm,细胞绿色荧光显示复合物组转染率远大于裸质粒对照组。结论 MPEG-PLGA/pEGFP-C3纳米胶囊复合物可有效提高质粒的转染率,可作为一种新型跨膜载体在临床与研究中应用。更多还原

【Abstract】 Objective To authenticate the application of MPEG-PLGA nanometer capsule in eucaryon plasmid transfection cell. Methods To dissolve the MPEG-PLGA in 4℃ double-distilled water,to add green fluorescent carrier and then to place in 37℃ attemperator to prepare MPEG-PLGA/DNA nanometer capsule after 30 minutes.The MPEG-PLGA/pEGFP-C3 nanometer capsule’s diameter was detected.The compound’s transfection efficiency by observing the expression of green fluorescent was decided. ResultsThe MPEG-PLGA/pEGFP-C3 nanometer capsule’s diameter was 28.3 nm and its transfection efficiency was higher than bare plasmid control group. Conclusion MPEG-PLGA/pEGFP-C3 nanometer capsule can raise plasmid’s transfection efficiency effectually and is neotype transmembrane carrier in clinical research.更多还原