【摘要】 目的:构建神经干细胞缺氧损伤实验模型并探讨阿魏酸对缺氧损伤的神经干细胞是否有保护作用。方法:选取培养第三代的新生SD大鼠神经干细胞进行不同浓度和时间缺氧培养,确定神经干细胞缺氧培养模型。将细胞分为对照组(常规培养)、缺氧组(缺氧8h后)、加药组(缺氧8h+不同浓度的阿魏酸5g/ml、50g/ml、500g/ml)。MTT法测定各组细胞活性,免疫组织化学检测Nestin及血清诱导分化后NSE和GFAP的表达。结果:95%N2,5%CO2培养8h能成功构建离体神经干细胞缺氧损伤模型;不同浓度的阿魏酸能对缺氧损伤的神经干细胞发挥保护作用且呈剂量依赖关系。结论:阿魏酸对缺氧状态下神经干细胞有保护作用,其机制可能与促进神经干细胞增殖和诱导其分化作用有关。更多还原

【Abstract】 Objective: To establish hypoxia damage model of neural stem cells(NSCs) and investigate the protection of ferulic acid on them.Methods: NSCs were cultured from neonatal mice;immunocytochemistry was used to examine the Nestin expression of NSC;the third passage cells were divided into control,hypoxia and ferulic acid groups,NSE and GFAP was used to evaluate cell differentiation,MTT was used to evaluate cell proliferation,and NSE and GFAP was used to evaluate cell differentiation.Results: NSCs under hypoxia(95% N2,5%CO2)for 8 hours can establish hypoxia damage model in vitro;FA can protect NSCs under hypoxia and this function was dose-dependent.Conclusion: FA may prevent NSCs against hypoxia injury,the mechanism may be that it has something to do with NSCs proliferation and differentiation.更多还原