【摘要】 目的:从动力学角度分析乙醛刺激大鼠肝星状细胞活化的时间变化,以确定大鼠肝星状细胞活化的最佳时间,从而为后续肝纤维化的相关研究提供实验数据。方法:培养大鼠肝星状细胞,同步化处理12h,随机分为乙醛刺激组和对照组。其中乙醛刺激组细胞加入乙醛(终浓度200μmol/L)分别刺激12h、24h和36h,每12h补加一次乙醛;对照组加入等量培养基。采用MTT法测定各组细胞增殖;ELISA法检测Ⅰ型胶原蛋白、纤维黏连素;Western blotting检测α-SMA的表达。结果:加入乙醛后,细胞的增殖活性随时间的延长而增强,24h细胞增殖活性达最高峰;ELISA测定结果显示,乙醛刺激组较对照组有明显上调;Western blotting检测结果,乙醛刺激组明显促进α-SMA表达量上调,且在24h达高峰。结论:乙醛可激活大鼠肝星状细胞且活化的最佳作用时间为24h。更多还原

【Abstract】 Objective: To choose the best active time of rat hepatic stellate cell stimulated by acetaldehyde.Methods:The rat hepatic stellate cells lines HSC-T6 were cultured in a humidified incubator and were divided into control and test groups.The test groups were stimulated by acetaldehyde(200μmol/L) at 12h、24h and 36h.The HSC proliferation was detected by MTT,the expression of TIMP Ⅰand FN was detected by ELISA,and the protein levels of α-SMA were detected by Western blotting.Results: Acetaldehyde stimulation induced HSC-T6 activation.Acetaldehyde could up-regulate the expression of TIMPⅠand FN,and also up-regulate α-SMA expression in HSC-T6,which were maximum at 24h.Conclusion:The best activation time of HSC-T6 stimulated by acetaldehyde is at 24h.更多还原