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白花玉簪的组培快繁技术研究

Tissue Culture and Rapid Propagation of the Hosta plantaginea Aschers

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【作者】 姜冬

【Author】 JIANG Dong(The Forest Management Institute of Liaoning Provice, Dandong, Liaoning 118002)

【机构】 辽宁省森林经营研究所

【摘要】 对白花玉簪的组织培养技术进行了研究。结果表明:以休眠芽做外植体培养在MS+BA 1.0mg/L+NAA 0.1 mg/L培养基上可诱导增殖出苗,系数5倍以上;生根培养以1/2MS+IBA 0.5 mg/L培养基为最好,生根率达90%,;移栽基质以纯沙为最好,成活率达80%以上。

【Abstract】 Cell culture was made of the Hosta plantaginea Aschers for its rapid propagation。 Orthogonal design indicated that MS+6-BA 1 mg/L+NAA 0.1 mg/L was the best culture medium to induce proliferation buds from its tuber. Propagation coefficient was five times. The best culture medium for multiplication of cluster shoots was1/2 MS + IBA 0.5 mg/L. When micropropagated plantlets with well-developed root systems were transferred to planting bed of sand in a greenhouse, 80% of the plantlets survived.

  • 【文献出处】 辽宁农业科学 ,Liaoning Agricultural Sciences , 编辑部邮箱 ,2012年04期
  • 【分类号】S567.239
  • 【被引频次】3
  • 【下载频次】108
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