【摘要】 提出了毛细管电泳法同时分离测定中药萹蓄中山柰素、槲皮素和咖啡酸含量的方法,以pH 8.5的20mmol.L-1硼酸钠-磷酸二氢钠为缓冲溶液,运行电压20kV,重力进样时间14s,在254nm波长处进行紫外检测。山柰素、槲皮素和咖啡酸在7min内即可实现分离。3者的线性范围依次为0.50～100,0.70～146,1.0～124mg.L-1,检出限(3S/N)依次为0.3,0.4,0.6mg.L-1。采用此法对萹蓄样品进行了分析,所得加标回收率在95.3%～106%之间,测定值的相对标准偏差(n=5)均小于3.5%。更多还原

【Abstract】 A method for the simultaneous separation and determination of kaempferol,quercetin and caffeic acid in the TCM,Polygonum aviculare by capillary electrophoresis was proposed.Mixed solution of 20 mmol·L-1 Na2B4O7-NaH2PO4 of pH 8.5 was used as the buffer solution,moving voltage of 20 kV and gravity injection time of 14 s were applied.UV-detection at the wavelength of 254 nm was adopted.The 3 analytes were completely separated within 7 min.Linearity ranges between 0.50-100(for kaempferol),0.70-146(for quercetin) and 1.0-124 mg·L-1(for caffeic acid) were obtained,with detection limits(3S/N) of 0.3,0.4 and 0.6 mg·L-1 respectively.The proposed method was applied to the analysis of sample of Polygonum aviculare,giving values of recovery obtained by standard addition method in the range of 95.3%-106% with RSD′s(n=5) less than 3.5%.更多还原