【摘要】 提出了高效液相色谱法测定平胃丸中橙皮苷含量的方法。采用Hypersil BDS C18(200mm×4.6mm,5μm)色谱柱;以甲醇-乙酸-水(35+4+61)混合溶液为流动相,流量1mL.min-1,检测波长为283nm,橙皮苷的质量浓度与峰面积在8.08～121.2mg.L-1范围内呈线性关系。方法用于平胃丸中橙皮苷的测定,平均回收率为101.1%,相对标准偏差(n=9)为1.2%。更多还原

【Abstract】 HPLC was applied to the determination of hesperidin in the prepared tradition Chinese medicine named "Pingwei Wan".The Hypersil BDS C18 column(250 mm×4.6 mm,5 μm) was used as stationary phase,and a mixture of methanol,acetic and water,mixed in the ratio of 35 to 4 to 61 by volume,was used as mobile phase with a flow-rate of 1 mL·min-1.Detection at the wavelength of 283 nm was adopted in the determination.Linear relationship between values of peak area and mass concentration of hesperidin was obtained in the range of 8.08-121.2 mg·L-1.The proposed method was used in the determination of hesperidin in the drug,giving average recovery of 101.1% with RSD(n=9) 1.2%.更多还原