【摘要】 目的评价核酸类似物PNA在HepG2.2.15细胞中对乙型肝炎病毒复制的抑制作用。方法以HepG2.2.15细胞作为细胞模型,拉米夫定作为阳性对照药物。HepG 2.2.15细胞于药物处理14 d后,收集上清液及细胞。采用ELISA检测上清液中HBsAg和HBeAg含量,HBV荧光定量检测上清液和细胞内HBV DNA水平,CCK-8试剂盒检测药物对细胞的毒性作用。结果核酸类似物PNA与拉米夫定在浓度1.6～1 000μmol·L^-1内对HepG 2.2.15细胞的毒性均较小。与药物未处理组比较,PNA和拉米夫定均可有效抑制细胞上清HBV DNA,半数抑制浓度(IC₅₀)分别为0.05～0.10μmol·L^-1和0.09～0.18μmol·L^-1,两者之间差异无统计学意义。结论在体外细胞实验中,PNA对细胞的毒性小,与拉米夫定的安全指数相当;PNA对乙型肝炎病毒复制有较强的抑制作用,且具有一定时效量效关系,与拉米夫定的抑制强度相当。更多还原

【Abstract】 AIM To study the inhibitory effects of hepatiotis B virus（HBV） replication by PNA in HepG 2.2.15 cell line.METHODS Both positive drug lamivudin（3TC） and PNA were added in HepG 2.2.15 cells for 14 d respectively. Cell Counting Kit - 8 was used to detect the cell proliferation and drug toxicity.ELISA kit detected HBsAg and HBeAg in supernatant.HBV DNA copies were quantized by using HBV PCR quantitative fluorescence detection kit.RESULTS Both PNA and 3TC had little drug toxicity in HepG 2.2.15 cell line.Comparing to negative control,PNA could effectively reduce HBV DNA levels in supernatant.The ranges of IC₅₀ for PNA were 0.05 - 0.10μmol·L^-1,whereas the ranges of IC₅₀ were 0.09 - 0.18μmol·L^-1 after 3TC treatment.These results indicated PNA had the same efficacy and safety as positive drug 3TC.CONCLUSION PNA has little drug toxicity in HepG 2.2.15 cell line and can inhibit HBV replication.There is no significant difference between PNA and 3TC.更多还原