【摘要】 目的建立PEG-PLGA纳米粒中银杏内酯B（GB）的包封率和载药量测定的HPLC-ELSD方法。方法色谱条件为:Shiseido C₈柱（250 mm×4.6 mm,5μm）;V（甲醇）:V（四氢呋喃）:V（水）=31:11:58为流动相;蒸发光散射检测器检测参数:蒸发口温度105℃,载气（N₂）流速1.2 L·min^-1,柱温45℃。应用低温超速离心法和凝胶过滤相结合的方法纯化纳米粒。结果本次建立的HPLC-ELSD方法在GB质量浓度50～500 mg·L^-1内线性关系良好,r=0.999 1;重复性试验RSD为4.28%;平均回收率为95%～105%;载GB的PEG-PLGA纳米粒中杂质对GB的测定无干扰。GB的包封率为75.33%,载药量为3.77%。结论该方法简单、快速、准确,可用于载GB的PEG-PLGA纳米粒中GB包封率和载药量的测定。更多还原

【Abstract】 AIM To establish a HPLC method with evaporative light scattering detector（ELSD）to determine the encapsulating efficiency and loading capacity of ginkgolide B（GB）in poly（ethyleneglycol）-poly（L-lactic-co-glycolic acid）（PEG-PLGA）nanoparticles.METHODS Chromatographic conditions were as follows:Shiseido Cg column（250 mm×4.6 mm,5μm）;V（methanol）:V（tetrahydrofuran）:V（water）= 31:11:58 as mobile phase.ELSD parameters were set as follows:evaporation temperature was 105℃,carrier gas（N₂）flow rate was 1.2 L·min^-1and column temperature was 45℃.Low temperature ultracentrifugation and gel filtration methods were used to purify nanoparticles.RESULTS In the range of 50-500 mg·L^-1,all plots were linear and the correlation coefficients were high than 0.999 0;the relative standard deviation（RSD）of the reproducibility was 4.28%;average recovery was in the range of 95%-105%.As the dosage of GB was 1 mg,the encapsulating efficiency（EE）and the loading capacity（LC）was75.33%and3.77%, respectively.CONCLUSION The method is simple,rapid,accurate and can be used for quality control of GB loaded nanoparticles.更多还原