【摘要】 目的:探讨人鼻咽癌(nasopharyngeal carcinoma,NPC)细胞株中表皮生长因子受体(epidermal growth factor receptor,EGFR)蛋白的表达水平与西妥昔单抗细胞抑制率的相关性。方法:用蛋白质印迹法检测人NPC细胞株CNE-1、CNE-2、HONE-1、C-666、SUNE-1、5-8F以及HNE-1共7种细胞株中EGFR蛋白的表达水平,用MTT法检测西妥昔单抗对细胞株的抑制率。结果:西妥昔单抗浓度为1500μg/mL时,EGFR蛋白表达水平与抑制率呈正相关(r=0.444,P=0.044),西妥昔单抗浓度增加至1800μg/mL时,两者呈负相关(r=-0.468,P=0.032)。结论:在NPC细胞株中,EGFR蛋白表达水平与西妥昔单抗的细胞抑制率之间无相关性,EGFR蛋白表达水平不能作为预测西妥昔单抗疗效的指标。更多还原

【Abstract】 Objective:To study the correlation between epidermal growth factor receptor(EGFR) expression and inhibition ratios of cetuximab in human nasopharyngeal carcinoma cell lines.Methods: The level of EGFR protein expression of several human nasopharyngeal carcinoma cell lines,including CNE-1,CNE-2,HONE-1,C-666,SUNE-1,5-8F and HNE-1 were detected by Western blotting.The cell inhibition ratios of cetuximab were measured using MTT method.Results: When the density of cetuximab was 1500μg/mL,there was significant positive correlation between EGFR protein expression and inhibition ratio of cetuximab(r=0.444,P=0.044).However,there was significant negative correlation when the density was 1800 μg/mL(r=-0.468,P=0.032).Conclusions: There is no correlation between EGFR protein expression and inhibition ratios of cetuximab,and the level of EGFR protein expression can’t serve as a marker in predicting the therapeutic effect of cetuximab.更多还原