【摘要】 目的研究表皮生长因子受体(EGFR)、血管内皮生长因子(VEGF)及增殖细胞核抗原(Ki-67)在胃肠间质瘤(gastrointestinal stromal tumors,GIST)中的表达,并分析其临床意义。方法采用免疫组织化学SP法检测138例GIST中EGFR、VEGF及Ki-67的表达情况,分析其临床意义及相互关系。结果 EGFR、VEGF及Ki-67在GIST中的阳性表达率分别为78.3%、61.6%和64.5%。EGFR的表达与肿瘤的直径(χ2=8.387,P=0.004)、包膜的完整性(χ2=4.779,P=0.029)、风险分级(χ2=17.182,P=0.000)和发生部位(χ2=13.362,P=0.000)相关;VEGF的表达与肿瘤的直径(χ2=7.891,P=0.005)、包膜的完整性(χ2=8.962,P=0.003)及风险分级(χ2=24.280,P=0.000)相关;Ki-67的表达与肿瘤的直径(χ2=7.626,P=0.006)、包膜的完整性(χ2=4.924,P=0.026)及风险分级(χ2=9.859,P=0.002)相关。三者的表达均与患者的年龄及性别无关。EGFR、VEGF和Ki-67经分析显示两两之间均呈正相关。结论 EGFR、VEGF及Ki-67可能参与了GIST的发生、发展过程,并在此过程中相互协调,提示三者可作为评价GIST危险程度的重要指标。更多还原

【Abstract】 Objective To analyze the expression and clinical significance of EGFR,VEGF and Ki-67 protein in gastrointestinal stromal tumor(GIST).Methods Immunohistochemical staining of EGFR,VEGF and Ki-67 expression were conducted by streptavidin-peroxidase(SP) technique in 138 cases with gastrointestinal stromal tumor.The relationship between the expression and the clinicopathologic parameters was analyzed.Results The positive expression rate of EGFR,VEGF and Ki-67 in gastrointestinal stromal tumor were 78.3%,61.6% and 64.5% respectively.EGFR expression was correlated with tumor diameter(χ2=8.387,P=0.004),integrity of tumor envelope(χ2=4.779,P=0.029),risk grade(χ2=17.182,P=0.000) and tumor location(χ2=13.362,P=0.000);VEGF expression correlated with tumor diameter(χ2=7.891,P=0.005),integrity of tumor envelope(χ2= 8.962,P=0.003)and risk grade(χ2=24.280,P=0.000);Ki-67 expression correlated with tumor diameter(χ2=7.626,P=0.006),integrity of tumor envelope(χ2=4.924,P=0.026)and risk grade(χ2=9.859,P=0.002).There was no relationship with age and gender.The correlation of EGFR,VEGF and Ki-67 expressions showed positive correlation in any of the two.Conclusion EGFR,VEGF and Ki-67 may play an important role in the pathogenesis and progress of GIST and accommodate each other.The expression of EGFR,VEGF and Ki-67 can help to judge the risk degree of GIST.更多还原