【摘要】 目的:探讨Th17细胞相关因子白介素(IL)-23、IL-17A、IL-17F、IL-22与银屑病的关系与意义。方法:30例寻常性银屑病患者分为进行期15例,稳定期15例;30例正常人作为对照,取外周血,采用双抗体夹心ELISA法检测血浆IL-23的含量,实时荧光定量逆转录聚合酶链反应(FQ-RT-PCR)技术检测外周血中外周血单个核细胞(PBMC)IL-17A mRNA、IL-17FmRNA、IL-22 mRNA的相对表达量。结果:进行期银屑病患者血浆IL-23浓度、PBMC IL-17A mRNA、IL-17F mRNA、IL-22mRNA的表达均明显高于正常对照组,进行期患者PBMCIL-17A mRNA、IL-22 mRNA的表达高于静止期患者,而稳定期患者上述指标的表达与正常对照无统计学差异。结论:Th17细胞可能参与进行期寻常性银屑病病理过程。更多还原

【Abstract】 Objectives:To detect the expressions of Th17 cells related cytokines including IL-17A,IL-17F,IL-22 and IL- 23 in peripheral blood of patients with psoriasis vulgaris,and further illustrate the pathogenesis of psoriasis vulgaris. Methods:Peripheral blood was obtained from 15 patients with stable psoriasis vulgaris,15 patients with progressive psoriasis vulgaris and 30 healthy controls.The level of IL-23 in plasma was determined by ELISA,and the mRNA expression of IL-17A,IL-17F and IL-22 in peripheral blood mononuclear cell(PBMC) detected by FQ-RT-PCR(real time fluorescent quantitative-reverse transcriptase-polymerase chain reaction).Results:The level of IL-23 in progressive psoriasis was significantly higher than that of healthy control(P = 0.002),but this different was not found between the stable psoriasis patients and healthy control(P= 0.169).The levels of IL-17A mRNA,IL-17F mRNA and IL-22 mRNA in progressive psoriasis were significantly higher than those of healthy control(P = 0.007;P = 0.015;P = 0.002).The levels of IL-17A mRNA and IL-22 mRNA in progressive psoriasis were significantly higher than that in stable psoriasis other than IL-17F mRNA.Conclusions: Thl7 cells may take part in the pathogenesis of progressive psoriasis vulgaris.更多还原