【摘要】 目的探讨磷脂酶C-γ1(phospholipase C-γ1,PLC-γ1)对乳腺癌细胞生长、粘附和迁移等生物学行为的影响,揭示乳腺癌发生的分子机制。方法在乳腺癌细胞株MDA-MB-231中加入PLC-γ1抑制剂(U-73122),用MTT试验、划痕试验、细胞粘附及趋化运动试验检测细胞生物学行为的变化,western blot检测抑制PLC-γ1后的信号传导通路中整合素β1和PKCζ分子磷酸化激活的情况。结果 PLC-γ1被U-73122抑制后MDA-MB-231细胞的增殖、粘附和迁移运动能力下降,差异均有统计学意义(P<0.05)。western blot结果表明,细胞粘附相关整合素β1的磷酸化激活水平降低;趋化运动相关PKCζ磷酸化水平降低。结论 PLC-γ1参与调控乳腺癌细胞增殖、粘附和迁移运动,可能是乳腺癌分子诊断和靶向治疗的有效靶点。更多还原

【Abstract】 Objective To study the effects of phospholipase C-γ1 as a potential regulator on the biological behaviors of breast cancer cells,e.g.,proliferation,adhesion,migration,etc.,and explore the molecular mechanisms of breast cancer development.Methods Variation of PLC-γ1 expression level of human breast cancer cell line MDA-MB-231 was observed by adding PLC-γ1 inhibitor U-73122.The cellular biological behaviors were tested by MTT assay,scratch test,cellular chemotaxis and adhesion test.The level of phosphorylated integrin β1 and protein kinase C(PKC) ζ in the intracellular signal transduction pathways were detected by western blot.Results The proliferation,migration and adhesion of MDA-MB-231 cells decreased following the inhibition of MDA-MB-231 cells by PLC-γ1 inhibitor U-73122(P<0.05).The phosphorylation levels of integrin β1 and PKCζ reduced.Conclusion PLC-γ1 inhibition dampened proliferation,migration and adhesion of breast cancer cells,and might serve as an effective strategy for molecular diagnosis and targeted therapy of breast cancer.更多还原