【摘要】 目的研究柴胡皂苷对大鼠酒精性肝损害保护作用的机制。方法将60只雄性Wistar大鼠随机分为正常对照组、酒精性肝病模型对照组、柴胡皂甙低、中、高剂量组。通过长期酒精灌胃方法建立酒精性肝病大鼠模型,观察柴胡皂甙对大鼠血清AST、ALT活性和甘油三脂(TG)水平,以及肝组织超氧化物歧化酶(SOD)、谷胱甘肽(GSH)及谷胱甘肽过氧化物酶(GSH-PX)含量变化的影响。结果与正常对照组相比,模型组大鼠血清AST、ALT活性及TG含量明显升高(P<0.05),肝组织SOD、GSH及GSH-PX活性明显降低(P<0.05),与模型组及柴胡皂甙低剂量组比较,柴胡皂甙中、高剂量组血清AST、ALT活性及+TG含量明显降低(P<0.05),肝组织SOD、GSH及GSH-PX活性明显增高(P<0.05)。结论中、高剂量的柴胡皂苷能有效的改善大鼠酒精性肝损害造成的影响。更多还原

【Abstract】 Objective To explore the potential protective effects of the Bupleurum - derived saponin,saikoside,on alcohol - induced liver damage in a rat model.Methods A total of 60 male Wistar rats（weighing 180 -220 g） were randomly assigned to five equal groups;normal （control;no alcohol,no saikoside）,alcoholic liver disease(model;56% alcohol gavage of 10 ml · kg^-1 · d^-1 for eight weeks), model treated with 5 ml/kg（low dose）,10 ml/kg（mid dose）,and 15 ml/kg（high dose） of saikoside.Serum levels of aspartate aminotransferase （AST）,alanine aminotransferase（ALT）,and triglyeride（TG）,and activities of superoxide dismutase（SOD）,glutathione peroxidase（GSH -pX）,and GSH in the liver were measured and analyzed.Significance of intergroup differences was determined by the statistical t - test.Results Compared with the control group,the alcoholic liver disease model group had significantly higher serum levels of AST,ALT andTG（P < 0.05）,but significantly lower activities of SOD,GSH - pX and GSH in liver（P < 0.05）.Compared with the untreated model group and the low dose saikoside treatment group,the mid dose and high dose treatment groups had significantly lower serum levels of AST,ALT and TG（P< 0.05 ） and significantly higher activities of SOD,GSH - pX and GSH in the liver（P < 0.05）.Conclusion A sufficient does of saikoside（10-15 ml/kg body weight） can improve alcohol - induced perturbations in liver molecules related to lipid metabolism,lipid peroxidation,and inflammation in rats.更多还原