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双向电泳技术筛选乙肝患者血清中标志蛋白

Screening serum biomarkers of hepatitis B by two-dimensional electrophoresis technology

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【作者】 吴志鹏谢志勇林丽彬柯益彬李春来

【Author】 WU Zhi-peng,XIE Zhi-yong,LIN Li-bin,KE Yi-bin,LI Chun-lai(College of Medicine,Affiliated Hospital,Putian University,Putian 351100,China)

【机构】 莆田学院附属医院

【摘要】 利用双向电泳技术进行筛选乙肝患者血清中的标志蛋白,通过对比乙肝患者和健康人血清中蛋白质的双向电泳图谱,得到了11个显著的差异点,其中在乙肝患者血清中表达量上调有6个点,表达量下调有2个点;乙肝患者血清中新出现2个点,消失1个点。本研究为乙肝的诊断和治疗提供新的途径。

【Abstract】 Two-dimensional electrophoresis technology was employed to find out marked proteins in serum samples of hepatitis B patients.All 11 significant differences were found by comparing the 2-D gel maps of normal samples with hepatitis B samples.Compared with normal samples,6 up-regulated and 2 down-regulated spots were detected in the serum from hepatitis B patients.2 new spots were found only in hepatitis B samples and 1 new spot was found only in normal samples.The results might supply help for the diagnosis and treatment of hepatitis B.

【基金】 莆田学院科研启动基金项目,编号:2006Q014
  • 【文献出处】 黑龙江医药科学 ,Heilongjiang Medicine and Pharmacy , 编辑部邮箱 ,2012年01期
  • 【分类号】R512.62
  • 【被引频次】2
  • 【下载频次】136
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