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双向电泳技术筛选乙肝患者血清中标志蛋白
Screening serum biomarkers of hepatitis B by two-dimensional electrophoresis technology
【摘要】 利用双向电泳技术进行筛选乙肝患者血清中的标志蛋白,通过对比乙肝患者和健康人血清中蛋白质的双向电泳图谱,得到了11个显著的差异点,其中在乙肝患者血清中表达量上调有6个点,表达量下调有2个点;乙肝患者血清中新出现2个点,消失1个点。本研究为乙肝的诊断和治疗提供新的途径。
【Abstract】 Two-dimensional electrophoresis technology was employed to find out marked proteins in serum samples of hepatitis B patients.All 11 significant differences were found by comparing the 2-D gel maps of normal samples with hepatitis B samples.Compared with normal samples,6 up-regulated and 2 down-regulated spots were detected in the serum from hepatitis B patients.2 new spots were found only in hepatitis B samples and 1 new spot was found only in normal samples.The results might supply help for the diagnosis and treatment of hepatitis B.
【关键词】 双向电泳技术;
血清;
标志蛋白;
【Key words】 two-dimensional electrophoresis technology; serum; marked protein;
【Key words】 two-dimensional electrophoresis technology; serum; marked protein;
【基金】 莆田学院科研启动基金项目,编号:2006Q014
- 【文献出处】 黑龙江医药科学 ,Heilongjiang Medicine and Pharmacy , 编辑部邮箱 ,2012年01期
- 【分类号】R512.62
- 【被引频次】2
- 【下载频次】136