【摘要】 目的研究诱导型一氧化氮合酶(iNOS)在大鼠肺纤维化中的作用.方法在博莱霉素(BLM)致大鼠肺纤维化模型上,用免疫组化染色法测定肺组织iNOS的表达,硝酸还原酶法测定肺组织匀浆中NO含量,碱水法测定肺组织匀浆中HYP含量,HE染色法光镜观察肺组织形态学变化,评价iNOS在大鼠肺纤维化形成中的作用.结果肺组织NO含量:BLM组大鼠肺组织中NO含量高于对照组(P<0.05),L-NMMA组大鼠肺组织NO含量低于BLM组(P<0.05).肺组织HYP含量:BLM组大鼠肺组织中HYP含量高于对照组(P<0.05),L-NMMA组大鼠肺组织中HYP含量低于BLM组(P<0.05).肺组织iNOS表达:BLM组大鼠肺组织iNOS表达明显高于对照组(P<0.05);L-NMMA组大鼠肺组织iNOS表达低于BLM组(P<0.05).肺病理形态学:对照组大鼠肺组织结构正常;BLM组肺组织出现肺泡炎症、肺间质胶原纤维增生和肺纤维化;L-NMMA组大鼠肺部炎症和肺纤维化程度明显减轻.结论 iNOS抑制剂L-NMMA在一定程度能减轻肺炎症反应和肺纤维化程度,iNOS在肺纤维化中起着重要作用.更多还原

【Abstract】 Objective To study the role of inducible nitric oxide synthase in Bleomycin-induced pulmonary fibrosis in rats.Methods We used the pulmonary fibrosis model induced by bleomycin(BLM)to evaluate the role of iNOS in the formation process of pulmonary fibrosis in rats.The expression of iNOS in lung tissue was determined by the immunohistochemical staining method.The NO levels in the lung homogenates were measured by the nitrate reductase assay.The HYP content in lung homogenates was evaluated by the alkaline method.The lung tissue morphological changes were observed by light microscopy after HE staining.Results In the NO levels of the lung tissue,BLM group was higher than the control group(P<0.05)and L-NMMA group was lower than the BLM group(P<0.05).In the HYP levels in the lung tissue,BLM group was higher than the control group(P<0.05)and L-NMMA group was lower than the BLM group(P<0.05).In the iNOS expression of the lung tissue,BLM group was obviously higher than the control group(P<0.05)and L-NMMA group was lower than the BLM group(P<0.05).In the pathological morphology of the lung,the lung tissue was normal in rats in the control group,the lung tissue appeared alveolar inflammation,pulmonary interstitial collagen increase and pulmonary fibrosis in BLM group and the lung inflammation and pulmonary fibrosis in L-NMMA group was reduced significantly.Conclusion L-NMMA,a iNOS inhibitor,can reduce in a certain extent the inflammatory response of lung tissue and pulmonary fibrosis,suggesting iNOS plays an important role in the formation of pulmonary fibrosis更多还原