【摘要】 目的探讨依折麦布对db/db小鼠胰岛分泌功能的作用。方法将8周龄的db/db小鼠随机分为2组,分别给予6周依折麦布或安慰剂灌胃治疗,同周龄的db/m小鼠作为非糖尿病对照。投药6周后行糖耐量试验后取胰腺,进行胰岛素免疫组织化学检测,另采用离体胰岛灌流系统测定胰岛素第一时相的分泌。结果依折麦布组治疗6周后空腹血糖显著下降,同时总胆固醇及低密度脂蛋白胆固醇低于对照组(P<0.05)。糖负荷120 min后葡萄糖曲线下面积低于db/db对照组(P<0.05),AUCINS0-30高于对照组(P<0.05)。离体胰岛灌流结果显示依折麦布组胰岛第一时相分泌得到改善。胰岛内胰岛素表达强度依折麦布组明显高于对照组。结论依折麦布能改善糖耐量,恢复胰岛第一时相分泌,保护β细胞功能。更多还原

【Abstract】 Aim The aim of this study was to investigate whether ezetimibe has a protective effect on β-cell function in diabetic state. Methods 8-week-old db/db mice were randomized to ezetimibe 10 mg/(kg·d),or placebo.The age-matched nondiabetic littermates db/m mice were treated with placebo as the controls.After 6 weeks’ treatment,intraperitoneal glucose tolerance test and immunohistochemical staining for insulin were performed.Islet perifusion were performed to evaluate kinetics of insulin release by using islet perfusion system. Results Fasting blood glucose(FBG),total cholesterol(TC),low density lipoprotein cholesterol(LDLC) were lower in ezetimibe group than that in db/db group after 6 weeks’ treatment.Ezetimibe provided an improvement of glucose tolerance and greatly rescued islet β-cell mass.Ezetimibe treatment also improved first-phase insulin response. Conclusion Ezetimibe treatment effectively improved glucose tolerance and β-cell function by significantly restored first-phase insulin secretion on db/db mice.更多还原