【摘要】 抑制性差减杂交技术(suppression subtractive hybridization)是在差减杂交和抑制性PCR技术的基础上发展起来的鉴别差异表达基因的实验方法,用于分离2种具有相同或者相似遗传背景,但是表型上有差异的生物样品中差异表达的基因。这项技术在蚊虫许多研究领域,如抗药性、病原体感染、生理特性等得到广泛的应用。本文对抑制性差减杂交技术在蚊虫各研究领域的应用情况进行了综述。更多还原

【Abstract】 Suppression subtractive hybridization (SSH) is a technique based on subtractive hybridization and suppression PCR.SSH is an effective and sensitive way of identifying differently expressed genes in two samples with the same,or similar,genetic backgrounds but different phenotypes.This technique has been widely used in many areas of mosquito research,such as insecticide resistance,pathogenic infection,and physiology.Here we review progress in the application of SSH in these fields.更多还原