【摘要】 在室内用甲氧虫酰肼对甜菜夜蛾Spodoptera exigua(Hübner)幼虫进行抗药性选育,经过13代汰选,甜菜夜蛾抗性种群(Met-R)对甲氧虫酰肼的抗性倍数为4.19倍;交互抗性测定发现,Met-R甜菜夜蛾对毒死蜱、高效氯氰菊酯、虫螨腈、虫酰肼、甲胺基阿维菌素苯甲酸盐等药剂的交互抗性比值在1.05～2.07之间,不存在交互抗性。离体酶活性测定结果表明,Met-R甜菜夜蛾5龄幼虫中肠谷胱甘肽S-转移酶比活力是相对敏感种群(SS)甜菜夜蛾的2.44倍,存在显著性差异,说明谷胱甘肽S-转移酶活性增强可能与甜菜夜蛾对甲氧虫酰肼产生抗药性有关;但Met-R甜菜夜蛾全酯酶催化活性与SS的无显著性差异,说明其抗药性可能与酯酶无关。更多还原

【Abstract】 After 13 generations’ selection in the laboratory,the methoxyfenozide-selected strain (Met-R) of Spodoptera exigua (Hübner) showed only a 4.19-fold increase in resistance to methoxyfenozide.Met-R showed no cross-resistance to chlorpyrifos,beta-cypermethrin,chlorfenapyr,tebufenozide or emamectin benzoate,with resistance ratio increases of 1.05-2.07-fold.In vitro biochemical studies showed that GST activity in the midgut of 5th instar larvae of Met-R was 2.44 times greater than in larvae of the susceptible strain (SS),indicating that enhanced GST activity may be associated with the resistance of S.exigua to methoxyfenozide.In contrast,nonspecific-esterase activity in Met-R showed no significant difference from that in SS,suggesting that nonspecific-esterase may not be involved in the resistance.更多还原