【摘要】 目的评价Sysmex XE-5000血细胞分析仪体液模式对胸腔积液和腹水中肿瘤细胞的筛查作用。方法用Sysmex XE-5000血细胞分析仪体液模式测定91份乙二胺四乙酸二钾抗凝的胸腔积液和腹水标本,分析其研究参数中高荧光细胞比率及其散点图对胸腔积液和腹水中肿瘤细胞的筛查作用。采用SPSS11.5及Excel软件对相关数据进行统计分析。结果在肿瘤细胞阳性的标本中,高荧光细胞比率较阴性标本显著增高;散点图上,在高侧向荧光及高侧向散射光区域呈现细胞密集构成亮带的标本检出恶性肿瘤的可能性大。结论 Sysmex XE-5000血细胞分析仪体液模式所提供的高荧光细胞比率及其散点图可以提供肿瘤细胞的筛选信息以做进一步显微镜检查。更多还原

【Abstract】 Objective To evaluate the capability for detecting of tumor cells in ascitic and pleural fluids by Sysmex XE-5000 hematology analyzer.Methods 91 EDTA-K2 ascitic and pleural fluids samples were counted by Sysmex XE-5000 hematology analyzer.Highly fluorescent body fluid cells percent(HF-BF%) and scatter diagram were observed in the samples to evaluate the capability of detection of tumor cells.SPSS11.5 and Excel were used for statistical analysis.Results The samples with tumor cells showed increased Highly fluorescent body fluid cells percent(HF-BF%) compared with benign samples.Bright band composed by dense cells whose distribution was located at the zone with high side fluorescence lights(SFL) and side scattered lights(SSC) in scatter diagram provided information about the possible presence of tumor cells.Conclusion Highly fluorescent body fluid cells percent(HF-BF%) and distinguishing feature of scatter diagram can provide screening information about the presence of tumor cells.This suggests that such increase should lead to a manual differential review and an extensive examination of slides to check the presence of abnormal cells.更多还原