【摘要】 利用基因工程手段重组表达了牛肾来源的氨基酰化酶1(ACY1),并与实验室之前构建的N-乙酰鸟氨酸脱乙酰酶(NAO)进行比较,以N-乙酰-DL-蛋氨酸为底物,研究了其酶学性质,考察了pH、温度、反应时间、表面活性剂、金属离子等因素对拆分DL-蛋氨酸的影响。结果表明,重组NAO和ACY1拆分300 mmol/L的N-乙酰-DL-蛋氨酸所需时间分别是2 h和6 h;重组NAO的酶活及N-乙酰-L-蛋氨酸的转化率分别为1 139.41 U/g和98%,高于重组ACY1的671.24 U/g和58.78%,约是重组ACY1的1.7倍。更多还原

【Abstract】 Aminoacylase 1(ACY1) from bovine kidney was recombinantly expressed in E.coli BL21(DE3) with the vector pETDuet.The enzymatic properties of ACY1 and N-acetyl-ornithine deacetylase(NAO) were compared and studied.Several influencing factors of the enzyme reaction,such as pH,temperature,enzyme reaction time,surfactant and metal irons,were investigated.The results indicate that the resolution of 300 mmol/L N-Ac-DL-methionine with ACY1 needs 6 h,while that with NAO needs 2 h.The enzyme activity and conversion rate of NAO(1 139.41 U/g and 98%) are about 1.7 times higher than those of ACY1(671.24 U/g and 58.78%).更多还原