【摘要】 目的:研究大鼠坐骨神经缺损后背根神经节中长链非编码RNA(long noncoding RNA,lncRNA)的表达变化。方法:建立SD大鼠坐骨神经损伤0天、1天、4天和7天模型,取背根神经节组织进行lncRNA检测,进行差异基因的筛选,并应用实时荧光定量逆转录-多聚酶链反应(qRT-PCR)对芯片结果进行验证;利用lncRNA与mRNA特异性表达的标准化信号强度,构建基因共表达网络。结果:共筛选出24个显著下调的lncRNAs,qRT-PCR检测的lncRNA AF130881和AB020616的表达变化与芯片一致。得到与lncRNA AF130881和AB020616正向或负向共表达的蛋白编码基因25个。结论:大鼠坐骨神经缺损后背根神经节组织中lncRNA的表达谱发生改变。更多还原

【Abstract】 Objective: To analyze the expression of lncRNA in the dorsal root ganglia(DRGs) after resection of the sciatic nerve in rats.Methods: The expression of lncRNAs was examined in a sciatic nerve resection model at 0 d、1 d、4 d and 7 d by using an rat lncRNA array.The lncRNA expression was also confirmed by quantitative real-time polymerase chain reaction(qRT-PCR).Gene coexpression network was constructed according to the normalized signal intensity of specific expression lncRNAs and mRNAs.Results: A total of 24 lncRNAs were identified which were significantly down-regulated in the DRGs after resection from 0 through 1 and 4 until 7 d.The expression of lncRNA AF130881 and AB020616 analyzed by qRT-PCR was in agreement with the microarray data.The network was constructed from lncRNA AF130881 and AB020616 positively or negatively coexpressed with 25 coding mRNAs at the serial time points.Conclusion: This study demonstrated a different involvement of lncRNAs in the DRGs after resection of the sciatic nerve in a rat model.更多还原