【摘要】 目的对盐城地区2010年手足口病患儿及其临床重症病例进行病原学调查。方法采集手足口病患儿咽拭子和肛拭子,对其病毒核酸进行肠道病毒检测定型。结果 481例(含重症52例)患儿采集的咽拭、肛拭标本中,共检测出病毒核酸阳性326例,阳性率为67.78%,其中CA16型44例(13.50%)、EV71型179例(54.91%)、未定型103例(31.59%)。52例重症病例中共检测出病毒核酸阳性45例,阳性率为86.54%,其中EV71型32例(71.11%)、未定型13例(28.89%)、CA16型0例。326例病毒核酸阳性患儿中男214例,女112例;45例病毒核酸阳性重症患儿中男33例,女12例。结论盐城地区手足口病患儿检测到的主要病原体是CA16和EV71等,EV71感染率高于CA16感染率,肠道病毒EV71是引起重症病例的主要病原体。更多还原

【Abstract】 Objective To investigate the etiology of patients with hand-foot-and-mouth disease and clinical severe symptom in Yancheng area in 2010.Methods Enterovirus RNA with HFMD were isolated by collecting throat and rectal swabs from them and "Universal,EV71 and CA16 types" were detected using real-time fluorescent quantitative RT-PCR.Results Totally 481 patients(including 52 severe cases) collected throat swabs and rectal swabs,326 enterovirus RNA-positive samples were detected including 214 male and 112 female samples,with a positive detection rate of 67.78%.Among them,the positive samples of CA16 and EV71 were 44(13.50%) and 179(54.91%) respectively,while the untyped case was 103(31.59%).Forty-five enterovirus RNA-positive samples out of 52 clinically severe patients were tested including 33 male and 12 female patients,with a positive detection rate of 86.54%.Among these cases,32(71.11%) EV71-positive and 0 CA16-positive samples were identified and the untyped sample was 13(28.89%).Conclusion The hand-foot-and-mouth disease was basically caused by CA16 and EV71 in Yancheng area in 2010,the infection rate of EV71 was significantly higher than CA16,and EV71 was the major pathogen that could cause severe disease.更多还原