【摘要】 目的探讨红毛五加多糖(AGP)对人乳腺癌细胞系MDA-MB-231增殖以及对细胞核转录因子p53、c-myc表达的影响。方法在DMEM培养基内加入不同浓度AGP(0.15625、0.3125、0.625、1.25、2.5 g/L)体外培养人乳腺癌细胞系MDA-MB-231 1d、3d、5d、7d后,用细胞增殖及细胞毒性检测试剂盒WST-1测定人乳腺癌细胞的增殖,倒置显微镜观察其形态变化;用免疫荧光法、免疫印迹法检测人乳腺癌细胞凋亡相关蛋白p53、c-myc的表达。结果 WST-1法显示,不同浓度AGP作用3d、5d、7d后对人乳腺癌细胞系MDA-MB-231的增殖具有抑制作用,与阴性对照组相比差异有显著性(P<0.05);抑制率与AGP剂量、作用时间呈依赖性。免疫荧光法及免疫印迹法显示,AGP作用于人乳腺癌细胞系MDA-MB-231后,其细胞的c-myc表达明显减弱,并向细胞质内聚集;而免疫印迹法显示p53表达显著增强。结论 AGP具有抑制人乳腺癌细胞系MDA-MB-231增殖的作用,这可能与AGP下调c-myc表达、上调p53表达有关。更多还原

【Abstract】 Objective To study the effects of acanthopanax giraldii harms var hispidus hoo polysaccharides(AGP) on proliferation and expression of nuclear transcription factor p53 and c-myc in human breast cancer cell line MDA-MB-231.Methods The proliferation rate of human breast cancer cell line MDA-MB-231,which was incubated by different concentration of AGP(0.15625,0.3125,0.625,1.25,2.5g/L) in DMEM medium for 1,3,5,7 days in vitro respectively,was studied by WST-1 cell proliferation and cytotoxicity assay kit.The changes of human breast cancer cell line MDA-MB-231 morphology were observed by an inverted microscopy.The expression of apoptosis-related proteins such as p53 and c-myc was detected by fluorescence immunoassay and Western blotting.Results WST-1 assay showed that different concentration of AGP inhibited proliferation of human breast cancer cell line MDA-MB-231 treated for 3day,5day,7day when compared with the negative control group(P<0.05).The inhibition rate depended on AGP dose and reaction time.According to fluorescence immunoassay and Western blotting analysis,c-myc expression in cytoplasm reduced and p53 expression increased significantly after the treatment of AGP on human breast cancer cell line MDA-MB-231.Conclusion AGP has the effect of inhibiting proliferation on human breast cancer cell line MDA-MB-231.Its role may be related to reduce c-myc expression and increase p53 expression of human breast cancer cell line MDA-MB-231.更多还原