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利用农杆菌介导法获得转柠檬酸合成酶基因粳稻及其耐低磷的研究
Genetic Transformation of Japonica Rice Mediated with Agrobacterium tumefaciens Harboring the Citrate Synthase Gene and Tolerance of the Transgenic Plants to Low Phosphorus in Soil
【摘要】 利用根癌农杆菌介导法将柠檬酸合成酶CS基因导入吉林省主栽超级粳稻品种吉粳88中。经PCR检测,获得162株转基因阳性植株。转基因植株后代进一步经过PPT抗性筛选、分子检测和耐低磷筛选,获得5株(T3代)耐低磷性状明显且农艺性状较好的转基因植株。对转基因植株柠檬酸合成酶活性和柠檬酸含量的测定以及形态学和产量性状调查结果表明:转基因植株优于非转基因对照植株。
【Abstract】 The citrate synthase gene was transformed into main cultivar(super rice’Jijing 88’) of Jilin Province,mediated with Agrobacterium tumefaciens.Total 162 obtained transgenic plants(T0) were validated by PCR and southern blot.Five transgenic plants(T3) with tolerance to low phosphorus in soil and good agronomic characteristics were obtained by PPT selection,PCR and Southern blot confirmation and tolerance test to low phosphorus.The citrate synthase activity and citrate acid contents in root tips and leaves of the 5 transgenic plants were higher than the control.The growth and yield of the transgenic plants were superior to the control.
【Key words】 Japonica rice； Citrate synthase gene； Tolerance to low phosphorus； Genetic transformation；
- 【文献出处】 吉林农业科学 ,Journal of Jilin Agricultural Sciences , 编辑部邮箱 ,2012年06期
- 【分类号】S511.22
- 【被引频次】3
- 【下载频次】103