【摘要】 为建立一种准确、简便的蛋黄卵磷脂中磷脂酰胆碱的检测方法,采用高效液相色谱-紫外检测法对其进行了研究。SHIMADZU LC-2010高效液相色谱仪配备AGILENT ZORBAXRX-SIL型硅胶柱,在200nm下对磷脂酰胆碱进行检测,流动相采用简单的甲醇/乙酸二元体系,加入的乙酸溶液改善了磷脂酰胆碱的峰形,有效抑制了严重的拖尾现象。流速较低,为0.5mL/min,减少了流动相的使用量。采用外标法定量,标准曲线线性范围较宽,为10～160μg/mL。检出限为0.5904μg/mL,定量限为1.9680μg/mL。经方法学验证性试验,证明本方法精密度、稳定性良好,准确度较高。更多还原

【Abstract】 In order to establish an accurate,simple detection method of phosphatidylcholine of egg yolk lecithin,this research paid attention to high performance liquid chromatography(HPLC)-ultraviolet(UV) detector method.SHIMADZU LC-2010 HPLC equipped with AGILENT ZORBAX RX-SIL silica gel column was used.Detection wave was 200 nm.Simpler binary system of methanol and ethanoic acid was used as mobile phase,and added ethanoic acid improved phosphatidylcholine chromatograph peak shape,tail formation was inhibited efficiently.Flow rate was 0.5 mL/min.It was relatively small and reduced mobile phase usage.External standardization quantization was used,and the standard curve linear range was wide and it was 10 ～160 μg/mL.Detection limit was 0.5904 μg/mL,and quantitative limit was 1.9680 μg/mL.The validation test proved the proposed method is characterized by high precision and good stability.更多还原