【摘要】 目的探讨应用荧光定量PCR(FQ-PCR)技术在病理组织中检测结核分枝杆菌DNA(TB-DNA)的临床应用价值。方法对196例临床诊断结核的穿刺病理活检标本进行石蜡包埋切片,采用实时荧光定量PCR技术检测TB-DNA,并行抗酸染色及组织病理学检查,对三种检测与临床诊断符合率进行比较。结果在196例临床诊断结核的石蜡包埋组织中,荧光定量PCR检测阳性136例,阳性率为69.39%,抗酸染色检测阳性69例,阳性率为35.20%,组织病理学检查阳性102例,阳性率为52.04%,荧光定量PCR检测阳性率最高。结论荧光定量PCR技术简便、快捷,敏感性强、特异性高,可作为结核病分子病理诊断的重要检测方法,具有较高的临床应用价值。更多还原

【Abstract】 Objective To explore the application of fluorescent quantitative PCR(FQ-PCR) and its clinical value for histopathological detection of mycobacterium tuberculosis DNA(TB-DNA).Methods The paraffin-embedded needle biopsy sections from 196 cases with tuberculosis were detected by quantitative PCR technique with TB-DNA in real time,and acid-fast staining and histopathological examinations were conducted at the same time.Results Among 196 cases with the clinical diagnosis of tuberculosis in paraffin-embedded tissues,there were 136 cases(69.39%) screened positive by fluorescence quantitative PCR,69 cases(35.20%) screened positive by acid-fast staining examination,and 102 cases(52.04%) screened positive by histopathological examination.The fluorescence quantitative PCR detection is of the highest positive rate.Conclusion The fluorescence quantitative polymerase chain reaction technology is simple,efficient,and of sensitivity and high specificity.Since it is of high clinical value,it can be used as a pathological diagnostic method for tuberculosis molecular detection.更多还原