【摘要】 目的建立黔产蒲公英中熊果酸含量的高效液相色谱测定方法。方法采用DikmaC18柱(250mm×4.6mm,5μm),流动相为甲醇-乙腈-0.5%醋酸铵(16:65:20),柱温25℃,流速1.0mL·min-1,检测波长为215nm。结果熊果酸在10.2~102μg·mL-1范围内与峰面积呈良好的线性(r2=0.9999),平均加样回收率为99.52%,RSD为1.47%(n=6)。结论所用方法简便、快速、准确,首次提出以熊果酸含量作为黔产蒲公英的质量控制指标。更多还原

【Abstract】 Objective: To establish the content determination method of ursolic acid in Taraxacum mongolicum Hand.-Mazz by HPLC. Methods: Using HPLC to investigate the content determination. The HPLC system consisted of Dikma C18 (250 mm × 4.6 mm, 5 μm), mobile phase of methyl alcohol-acetonitrile-0.5% ammonium acetate (15:65:20) mixture, column temperature of 25℃ , flow rate of 1.0 mL/min, detection at 215 nm. Results: It was well linear between the concentrations from 10.2 to 102 μg/mL and the peak areas, the recovery rate was 99.52% (RSD = 1.47, n = 6). Conclusion:It is more suitable for ursolic acid as ground-level quality control for Taraxacum mongolicum.更多还原